Proteomics

Dataset Information

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Comprehensive biochemical characterization of Mycobacterium tuberculosis clonal strains after isoniazid resistance


ABSTRACT: This project describes the isoniazid (INH) resistance acquisition event in Mycobacterium tuberculosis (Mtb) from the proteomics perspective. In this way, an exploration of the protein differences, comparing clonal INH susceptible (INHs) and INHr pairs of Mtb were evaluated. One clonal clinical and one clonal laboratory-derived Mtb pair with different susceptibility profiles to INH were studied. The laboratory INHr strain had one katG mutation (V1A), while the clinical INHr strain had two (V1A and E3V). Large-scale bacterial cultures were grown in triplicate to obtain secreted proteins as well as proteins from cell fractions. The resulting peptide solutions from all fractions were analyzed using liquid-chromatography coupled with tandem mass spectrometry (LC-MS/MS). LC-MS/MS spectra were compared against an Mtb database to determine the protein abundance. Protein abundance differences were tested by Student’s t test. Looking at the same cellular fractions, there were 25 commonly altered Mtb proteins after acquiring INH resistance. These proteins were involved in ATP synthase machinery, lipid metabolism, regulatory events, virulence, detoxification and adaptation processes.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mycobacterium Tuberculosis

TISSUE(S): Prokaryotic Cell

DISEASE(S): Pulmonary Tuberculosis

SUBMITTER: Carolina Mehaffy  

LAB HEAD: Karen M Dobos

PROVIDER: PXD009549 | Pride | 2018-06-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CFP_21-1.mzid.gz Mzid
CFP_21-1.mzid_CFP_21-1.MGF Mzid
CFP_21-1.mzid_CFP_21-1.pride.mgf.gz Mzid
CFP_21-1.pride.mztab.gz Mztab
CFP_21-1.raw Raw
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Publications

Biochemical Characterization of Isoniazid-resistant <i>Mycobacterium tuberculosis:</i> Can the Analysis of Clonal Strains Reveal Novel Targetable Pathways?

Nieto R Luisa Maria LM   Mehaffy Carolina C   Islam M Nurul MN   Fitzgerald Bryna B   Belisle John J   Prenni Jessica J   Dobos Karen K  

Molecular & cellular proteomics : MCP 20180529 9


Tuberculosis (TB) continues to be an important public health threat worldwide, due in part to drug resistant <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) strains. The United States recently reported a shortage of isoniazid (INH), which could drive higher INH resistance rates. Changes in the <i>Mtb</i> proteome before and after acquisition of INH resistance in a clean genetic background remain understudied and may elucidate alternate drug targets. Here, we focused on <i>Mtb</i> clonal strains t  ...[more]

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