Proteomics

Dataset Information

0

MHC II immunopeptidome during Plasmodium berghei blood stage infection

ABSTRACT: In malaria, T cells play a dual role by both restricting parasite growth and mediating immunopathology such as the deadly neuroinflammation called cerebral malaria. During experimental cerebral malaria (ECM), IFN produced by CD4 T cells promotes CD8 T cell sequestration in brain capillaries, resulting in endothelial damage, oedema and death. However the antigen-presenting cells controlling the development of CD4 T cell responses, as well as the antigens recognized by these CD4 T cells, are unknown. Here we used mass spectrometry to characterize the MHC II immunopeptidome presented by dendritic cells during blood stage malaria in C57BL/6 mice. We identified 14 MHC II ligands derived from 13 conserved Plasmodium berghei proteins that we validated in vivo. This work profiles the first MHC II immunopeptidome in a mouse model of blood stage malaria.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Berghei Anka Mus Musculus (mouse)

TISSUE(S): Dendritic Cell, Blood

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: SALIOU Jean-Michel  

LAB HEAD: Jean-Michel Saliou

PROVIDER: PXD007628 | Pride | 2017-09-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
QE003777TA.raw Raw
QE003777TAF003988.mzid Mzid
QE003777TAF003988.mzid_QE003777TA_F003988.MGF Mzid
QE004485SJ.raw Raw
QE004485SJF003051.mzid Mzid
Items per page:
1 - 5 of 6
altmetric image

Publications

Profiling MHC II immunopeptidome of blood-stage malaria reveals that cDC1 control the functionality of parasite-specific CD4 T cells.

Draheim Marion M   Wlodarczyk Myriam F MF   Crozat Karine K   Saliou Jean-Michel JM   Alayi Tchilabalo Dilezitoko TD   Tomavo Stanislas S   Hassan Ali A   Salvioni Anna A   Demarta-Gatsi Claudia C   Sidney John J   Sette Alessandro A   Dalod Marc M   Berry Antoine A   Silvie Olivier O   Blanchard Nicolas N  

EMBO molecular medicine 20171101 11

In malaria, CD4 Th1 and T follicular helper (T<sub>FH</sub>) cells are important for controlling parasite growth, but Th1 cells also contribute to immunopathology. Moreover, various regulatory CD4 T-cell subsets are critical to hamper pathology. Yet the antigen-presenting cells controlling Th functionality, as well as the antigens recognized by CD4 T cells, are largely unknown. Here, we characterize the MHC II immunopeptidome presented by DC during blood-stage malaria in mice. We establish the i  ...[more]

Similar Datasets

Proteomics Interactome of the Autophagy Receptor TAX1BP1 (T6BP)
2022-09-22 | PXD024330 | Pride
Proteomics Immunopeptidome of HeLa-CIITA cells silenced for TAX1BP1(T6BP) expression
2022-09-22 | PXD024417 | Pride
Proteomics The pancreatic islet MHC-II peptidome identifies key features of autoimmune peptides
2020-03-12 | PXD015408 | Pride
Proteomics Adjuvant MHC-II Immunopeptidome and proteome
2024-10-30 | PXD052277 |
Proteomics MHC Immunopeptidome profiling of human coronavirus OC43-infected cells identifies CD4 T cell epitopes specific to seasonal coronaviruses and epitopes cross-reactive with SARS-CoV-2
2022-10-27 | MSV000090595 | MassIVE
Proteomics Machine learning predictions of MHC-II specificities reveal alternative binding mode of class II epitopes
2023-04-07 | PXD034773 | Pride
Transcriptomics Predominance of interferon-related responses in the brain during murine malaria as identified by microarray analysis.
2008-03-14 | E-GEOD-9808 | biostudies-arrayexpress
Transcriptomics Transcription profiling of genetically cerebral malaria-resistant (CM-R) and cerebral malaria-susceptible (CM-S) mouse strains
2005-06-30 | E-MEXP-370 | biostudies-arrayexpress
Proteomics Nano LC-MS/MS DDA mass spectrometry profiling of I-Ab MHCII immunopeptidome eluted from B6 mice on normal and high fructose/high fat (HFHF) diet
2022-08-22 | PXD026068 | Pride
Transcriptomics Expression data from self-deprived regulatory CD4 T cells
2011-01-20 | E-GEOD-26714 | biostudies-arrayexpress