Dataset Information

Serial-Omics of Equine Urine - Serial-omics characterization of equine urine

ABSTRACT: Horse urine is easily collected and contains molecules readily measurable using mass spectrometry that can be used as biomarkers representative of health, disease or drug tampering. This study aimed at analyzing microliter levels of horse urine to purify, identify and quantify proteins, polar metabolites and non-polar lipids. Urine from a healthy 12 year old quarter horse mare on a diet of grass hay and vitamin/mineral supplements with limited pasture access was collected for serial-omics characterization. The urine was treated with methyl tert-butyl ether (MTBE) and methanol to partition into three distinct layers for protein, non-polar lipid and polar metabolite content from a single liquid-liquid extraction and was repeated two times. Each layer was analyzed by high performance liquid chromatography – high resolution tandem mass spectrometry (LC-MS/MS) to obtain protein sequence and relative protein levels as well as identify and quantify small polar metabolites and lipids. The results show 46 urine proteins, many related to normal kidney function, structural and circulatory proteins as well as 474 small polar metabolites but only 10 lipid molecules. Metabolites were mostly related to urea cycle and ammonia recycling as well as amino acid related pathways, plant diet specific molecules, etc. The few lipids represented triglycerides and phospholipids. These data show a complete mass spectrometry based –omics characterization of equine urine from a single 333 uL mid-stream urine aliquot. The data can be used as a baseline for healthy urine composition and analyses can be used to monitor disease progression, health status, monitor drug use, etc.

INSTRUMENT(S): LTQ Orbitrap Elite, Q TRAP, Q Exactive

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Urine

SUBMITTER: John Asara

LAB HEAD: John M Asara

PROVIDER: PXD007646 | Pride | 2018-07-23

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	091516PSBSierraUrineLipid1.raw	Raw
	091516PSBSierraUrineLipid2.raw	Raw
	091516PSBSierraUrineLipid3.raw	Raw
	091516QSierraUrine1.wiff	Wiff
	091516QSierraUrine1.wiff.scan	Wiff

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Publications

Serial-omics characterization of equine urine.

Yuan Min M Breitkopf Susanne B SB Asara John M JM

PloS one 20171013 10

Horse urine is easily collected and contains molecules readily measurable using mass spectrometry that can be used as biomarkers representative of health, disease or drug tampering. This study aimed at analyzing microliter levels of horse urine to purify, identify and quantify proteins, polar metabolites and non-polar lipids. Urine from a healthy 12 year old quarter horse mare on a diet of grass hay and vitamin/mineral supplements with limited pasture access was collected for serial-omics charac ...[more]

PMID: 29028822

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