Dataset Information

Identification and PTPs in Anncaliia algerae

ABSTRACT: This project is focused on isolation and characterization of polar tube proteins (PTPs) of Anncaliia algerae using diverse bioinformatic and experimental methodologies - from mass spectrometry to heterologous expression to antibody production and immunolocalization. A novel protein was isolated from A. algerae polar tube extracts named PTP6 which could participates in recognition of host cell receptors as well.Moreover the obtained data on immunolabeling of the polar tubes in the process of extrusion, strongly support one of three hypothetical mechanisms for spore firing, namely the “everting-like-a-glove finger” model. The practical result of this fundamental research is the elaboration of the novel workflow that allows identification and isolation of PTPs in a given microsporidian species wich unites different approaches, such as homology search, sporal protein extractions by thiol-reducing agents, antibody-based approaches, as well as a protocol for purification of extruded polar tubes combined with mass spectrometry to identify new polar tube components.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Anncaliia Algerae

TISSUE(S): Fungal Asexual Spore

SUBMITTER: Didier VIALA

LAB HEAD: Frédéric DELBAC

PROVIDER: PXD040773 | Pride | 2023-07-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	E-PP-IHP-7970-F21_TP_Aa_190222104608-01.msf	Msf
	E-PP-IHP-7970-F21_TP_Aa_190222104608.mzML	Mzml
	E-PP-IHP-7970-F21_TP_Aa_190222104608.raw	Raw
	E-PP-IHP-7971-200kDa_ET-01.msf	Msf
	E-PP-IHP-7971-200kDa_ET.mzML	Mzml

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Publications

Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae.

Fayet Maurine M Prybylski Nastasia N Collin Marie-Laure ML Peyretaillade Eric E Wawrzyniak Ivan I Belkorchia Abdel A Akossi Reginald Florian RF Diogon Marie M El Alaoui Hicham H Polonais Valérie V Delbac Frédéric F

Scientific reports 20230530 1

Microsporidia are obligate intracellular parasites able to infect a wide range of hosts from invertebrates to vertebrates. The success of their invasion process is based on an original organelle, the polar tube, which is suddenly extruded from the spore to inoculate the sporoplasm into the host cytoplasm. The polar tube is mainly composed of proteins named polar tube proteins (PTPs). A comparative analysis allowed us to identify genes coding for 5 PTPs (PTP1 to PTP5) in the genome of the microsp ...[more]

PMID: 37253964

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