Proteomics,Multiomics

Dataset Information

0

Cell Surface Capture of mouse PSC-derived Cardiac Progenitor Cells


ABSTRACT: The identification of cell surface proteins on stem cells or stem cell derivatives is a key strategy for the functional characterization, isolation, and understanding of stem cell population dynamics. Here, using an integrated mass spectrometry and microarray based approach, we analyzed the surface proteome and transcriptome of cardiac progenitor cells (CPCs) generated from the stage-specific differentiation of mouse and human pluripotent stem cells. Through bioinformatic analysis, we have identified and characterized FZD4 as a new marker for lateral plate mesoderm. Additionally, we utilized FZD4, in conjunction with FLK1 and PDGFRA, to further purify CPCs and increase cardiomyocyte (CM) enrichment in both mouse and human systems. Moreover, we have shown that NORRIN presented to FZD4 further increases CM output via proliferation through the canonical WNT pathway. Taken together, these findings demonstrate a role for FZD4 in mammalian cardiac development.

OTHER RELATED OMICS DATASETS IN: PRJNA401864

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Peter Zandstra  

LAB HEAD: Peter Zandstra

PROVIDER: PXD007684 | Pride | 2018-01-03

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
OT1_2011-07-05_CY106_d0.pepxml Pepxml
OT1_2011-07-05_CY106_d0.protxml Other
OT1_2011-07-05_CY106_d0.raw Raw
OT1_2011-07-05_CY109_d375.pepxml Pepxml
OT1_2011-07-05_CY109_d375.protxml Other
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Publications

FZD4 Marks Lateral Plate Mesoderm and Signals with NORRIN to Increase Cardiomyocyte Induction from Pluripotent Stem Cell-Derived Cardiac Progenitors.

Yoon Charles C   Song Hannah H   Yin Ting T   Bausch-Fluck Damaris D   Frei Andreas P AP   Kattman Steven S   Dubois Nicole N   Witty Alec D AD   Hewel Johannes A JA   Guo Hongbo H   Emili Andrew A   Wollscheid Bernd B   Keller Gordon G   Zandstra Peter W PW  

Stem cell reports 20171214 1


The identification of cell surface proteins on stem cells or stem cell derivatives is a key strategy for the functional characterization, isolation, and understanding of stem cell population dynamics. Here, using an integrated mass spectrometry- and microarray-based approach, we analyzed the surface proteome and transcriptome of cardiac progenitor cells (CPCs) generated from the stage-specific differentiation of mouse and human pluripotent stem cells. Through bioinformatics analysis, we have ide  ...[more]

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