Proteomics

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Human cWAF LC-MS/MS

ABSTRACT: Chronic skin wounds accompany many widespread, chronic and age-related diseases and are a major cause of morbidity and mortality. Both, keratinocytes and skin fibroblasts contribute to the pathomechanisms observed in chronic wounds. Whereas dysregulated pathways in the epidermis have been extensively studied, little is known on the influence of dermal fibroblasts on chronic wounding. We isolated fibroblasts from chronic wounds and show that they are a distinct cell type that can be propagated in vitro. Chronic wound-associated fibroblasts (cWAFs) exhibit a unique proteome profile characteristic for a decreased propensity for cell proliferation and migration but with an enhanced ability to contract the extracellular matrix. Commonly, they display lysosomal dysfunction and dysregulated TGFbeta signaling. Here, we define intrinsic and extrinsic properties of cWAFs that may contribute to pathological wound healing.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin, Fibroblast

DISEASE(S): Wounds And Injuries

SUBMITTER: Joern Dengjel  

LAB HEAD: Joern Dengjel

PROVIDER: PXD007704 | Pride | 2020-10-29

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20150202_BB_WAF1_Exp1_01.RAW Raw
20150202_BB_WAF1_Exp1_02.RAW Raw
20150202_BB_WAF1_Exp1_03.RAW Raw
20150202_BB_WAF1_Exp1_04.RAW Raw
20150202_BB_WAF1_Exp1_05.RAW Raw
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Publications

Proteomic Profiling of Fibroblasts Isolated from Chronic Wounds Identifies Disease-Relevant Signaling Pathways.

Berberich Bettina B   Thriene Kerstin K   Gretzmeier Christine C   Kühl Tobias T   Bayer Hans H   Athanasiou Ioannis I   Rafei-Shamsabadi David Ali DA   Bruckner-Tuderman Leena L   Nyström Alexander A   Kiritsi Dimitra D   Dengjel Jörn J  

The Journal of investigative dermatology 20200417 11

Chronic skin wounds accompany many prevalent age-related diseases and are a major cause of morbidity and mortality. Both keratinocytes and fibroblasts contribute to the pathomechanisms in chronic skin wounds. Dysregulated pathways in the epidermis have been extensively studied, but little is known of the influence of dermal fibroblasts on chronic wounding. We isolated fibroblasts from chronic wounds, propagated them in vitro, and analyzed them using proteomic profiling in combination with functi  ...[more]

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