Proteomics

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The role of the sRNA GcvB in Pasteurella multocida protein production

ABSTRACT: In this study, transcriptomic analysis of the P. multocida strain VP161 revealed a putative sRNA with high identity to GcvB from Escherichia coli and Salmonella enterica serovar Typhimurium. High-throughput quantitative liquid proteomics was used to compare the proteomes of the P. multocida VP161 wild-type strain, a gcvB mutant and a GcvB overexpression strain. These analyses identified 47 proteins that displayed significant differential production after inactivation of gcvB, 37 of which showed increased production. Thus, GcvB predominantly acts to negatively regulate protein production in P. multocida. Of the 37 proteins that were repressed by GcvB, 27 were predicted to be involved in amino acid biosynthesis or transport. Bioinformatic analyses of putative P. multocida GcvB target mRNAs identified a strongly conserved 10 nucleotide consensus sequence, 5’‑AACACAACAT-3’, with the central eight nucleotides identical to the seed binding region present within GcvB mRNA targets in E. coli and S. Typhimurium.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pasteurella Multocida

SUBMITTER: Ralf Schittenhelm  

LAB HEAD: John D. Boyce

PROVIDER: PXD007719 | Pride | 2019-11-12

REPOSITORIES: Pride

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Determination of the small RNA GcvB regulon in the Gram-negative bacterial pathogen <i>Pasteurella multocida</i> and identification of the GcvB seed binding region.

Gulliver Emily L EL   Wright Amy A   Lucas Deanna Deveson DD   Mégroz Marianne M   Kleifeld Oded O   Schittenhelm Ralf B RB   Powell David R DR   Seemann Torsten T   Bulitta Jürgen B JB   Harper Marina M   Boyce John D JD   Boyce John D JD  

RNA (New York, N.Y.) 20180212 5

<i>Pasteurella multocida</i> is a Gram-negative bacterium responsible for many important animal diseases. While a number of <i>P. multocida</i> virulence factors have been identified, very little is known about how gene expression and protein production is regulated in this organism. Small RNA (sRNA) molecules are critical regulators that act by binding to specific mRNA targets, often in association with the RNA chaperone protein Hfq. In this study, transcriptomic analysis of the <i>P. multocida  ...[more]

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