Dataset Information

The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of SMC4 involved in replicative senescence

ABSTRACT: In this study, a label-free quantitative proteomic approach was employed to analyze the serial passaged human skin fibroblast （CCD-1079Sk） cells, within 3305 proteins quantified. Of which, 372 proteins were significantly changed in early passage(P6), middle passage (P12) and later passage (P21), with a time-dependent decrease or increase tendency. Then, of which, SMC4 was selected for further biological validation. The results confirmed that the expression of SMC4 was significantly down-regulated in a time-dependent manner in the subculture of human skin fibroblasts (HSFb) with Western Blot experiment.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Elite

ORGANISM(S): Acyrthosiphon Pisum (pea Aphid) Homo Sapiens (human)

TISSUE(S): Skin, Bile

SUBMITTER: MENG QIAN

LAB HEAD: zhou hu

PROVIDER: PXD007732 | Pride | 2018-10-22

REPOSITORIES: Pride

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			Action	DRS
	20151213_mq_1079sk_p1-1_4h.raw	Raw
	20151213_mq_1079sk_p1-2_4h.raw	Raw
	20151213_mq_1079sk_p1-3_4h.raw	Raw
	20151213_mq_1079sk_p1-4_4h.raw	Raw
	20151213_mq_1079sk_p1-5_4h.raw	Raw

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The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence.

Meng Qian Q Gao Jing J Zhu Hongwen H He Han H Lu Zhi Z Hong Minhua M Zhou Hu H

Biochemical and biophysical research communications 20181015 4

Dermal fibroblast is one of the major constitutive cells of skin and plays a central role in skin senescence. The replicative senescence of fibroblasts may cause skin aging, bad wound healing, skin diseases and even cancer. In this study, a label-free quantitative proteomic approach was employed to analyzing the serial passaged human skin fibroblast (CCD-1079Sk) cells, resulting in 3371 proteins identified. Of which, 280 proteins were significantly changed in early passage (6 passages, P6), midd ...[more]

PMID: 30336977

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