Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pulsed SILAC labeling of the kidney

ABSTRACT: We microdissected kidneys into inner medulla, outer medulla inner stripe, outer medulla outer stripe and cortex.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD007749 | Pride | 2022-06-02

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20160603_UC_ColID_160_Markus_Rinschen_Benzing_0089_1600421MaRi_01.raw	Raw
	20160603_UC_ColID_160_Markus_Rinschen_Benzing_0089_1600421MaRi_02.raw	Raw
	20160603_UC_ColID_160_Markus_Rinschen_Benzing_0089_1600421MaRi_03.raw	Raw
	20160603_UC_ColID_160_Markus_Rinschen_Benzing_0089_1600421MaRi_04.raw	Raw
	20160603_UC_ColID_160_Markus_Rinschen_Benzing_0089_1600421MaRi_05.raw	Raw

Items per page:

1 - 5 of 72

Similar Datasets

Proteomic and phosphoproteomic landscape of kidney in rhesus macaques with SARS-CoV-2 infection

Project description:Six rhesus monkeys were divided into a control group (n=3) and a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection group (n=3). Meanwhile, their kidneys were further segmented into three regions: renal cortex (Cor), inner medulla (IM), and outer medulla (OM), and liquid chromatography-tandem mass spectrometry-based proteomics and phosphoproteomic profiling were conducted on these regions. Next, we conducted a comprehensive bioinformatics analysis, including differential analysis, functional enrichment, kinase prediction and potential drug target prediction.

2024-09-23 | PXD056118 |

Gene expression in the normal adult human kidney

Project description:This set of arrays was published in Molecular Biology of the Cell. The manuscript was released electronically on 12/01/2003 and the hard copy version in February 2004. The FT number indicates the kidney from which the component parts were dissected. Abstract: The kidney is a highly specialized organ with a complex, stereotyped architecture and a great diversity of functions and cell types. Because the microscopic organization of the nephron, the functional unit of the kidney, has a consistent relationship to the macroscopic anatomy of the kidney, knowledge of the characteristic patterns of gene expression in different compartments of the kidney could provide insight into the functions and functional organization of the normal nephron. We studied gene expression in dissected renal lobes of five adult human kidneys using cDNA microarrays representing approximately 30,000 different human genes. Total RNA was isolated from sections of the inner and outer cortex, inner and outer medulla, papillary tips, and renal pelvis and from glomeruli isolated by sieving. The results revealed unique and highly distinctive patterns of gene expression for glomeruli, cortex, medulla, papillary tips, and pelvic samples. Immunohistochemical staining using selected antisera confirmed differential expression of several cognate proteins and provided histological localization of expression within the nephron. The distinctive patterns of gene expression in discrete portions of the kidney may serve as a resource for further understanding of renal physiology and the molecular and cellular organization of the nephron An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed

2005-12-29 | E-GEOD-3931 | biostudies-arrayexpress

Gene expression in the normal adult human kidney

2005-12-30 | GSE3931 | GEO

Genetic deletion of Pax2 and Pax8 in adult mice

Project description:Background: The mammalian kidneys maintain salt and water homeostasis for proper electrolyte balance and hydration. As the glomerular filtrate passes through the nephron and into the renal medulla, electrolytes, water, and urea are reabsorbed through the concerted actions of solute carrier channels and aquaporins located at various positions along the nephron and in the outer and inner medulla. Renal epithelial cells develop from Pax2 positive proliferating stem cells that suppress Pax2 expression once differentiated into mature proximal and distal tubules, but continue to express the related Pax8 protein. The collecting tubules and renal medulla are derived from a Pax2 positive ureteric bud epithelia that continue to express Pax2 and Pax8 in adult kidneys. Despite the necessity for Pax2 in renal development, functions for Pax2 or Pax8 in adult renal epithelia have not been established. Methods: In this report, we deleted either Pax2, Pax8, or both genes in adult mice and examined the phenotypes and changes in gene expression patterns. The mechanism of Pax8 mediated activation of potential target genes was described in inner medullary collecting duct cells. Results: Mice with induced deletions of both Pax2 and Pax8 exhibit severe polyuria that can be attributed to significant changes in the expression of solute carriers, such as the urea transporter UTA1, and aquaporins within the inner and outer medulla. Furthermore, Pax8 expression is induced by high salt in collecting duct cells and activates the UTA1 gene by recruiting a histone methyltransferase complex to the promoter. Conclusions: These data uncover novel functions for Pax proteins, in adult renal epithelia, that are essential for retaining water and concentrating urine.

2019-12-05 | GSE141455 | GEO

Kidney inner medulla from mice treated with or without cisplatin

Project description:We are interested in the inner medulla of the kidney and how the nephrotoxic drug, cisplatin, may affect the transcriptome. Furthermore, we are interested in male and female inner medullary transcriptomic differences.

2023-01-01 | GSE195786 | GEO

mRNA-Seq profiling of human developing kidney

Project description:To characterize the molecular features of human kidney development, and to capture timing of emergence of important kidney compartments, we generated mRNA-Seq data from human kidneys at various developing stages. In order to capture tissue representing the entire kidney, we sampled kidneys with a wedge spanning from the cortex to medulla region.

2018-02-14 | GSE100859 | GEO

Assessment of ‘molecular organ age’ in pre-transplant kidney biopsies

Project description:Clinical kidney biopsies variably contain cortex and medulla depending on biopsy depth and angle. Therefore, biopsy composition may alter the transcriptional profile and confound conclusions drawn from differential gene expression analysis. To account for this in retrieval biopsies, we assessed differences in gene expression between paired cortex and medulla samples in n=5 human kidneys.

2024-08-16 | GSE222868 | GEO

Effect of potassium-depleted diet on kidney

Project description:Libraries obtained from microdissected kidney outer medullary collecting duct from C57Bl6/J mice control or after 3 days potassium-depleted diet. Keywords: other

2004-05-30 | GSE1445 | GEO

SADE analysis of microdissected kidney

Project description:Libraries generated from microdissected kidney samples from the healthy pole of cancerous kidneys. Donors were from either sex. Libraries were generated using Sau3A I as anchoring enzyme. Data are provided after removal of linker derived sequences.

2003-11-14 | GSE694 | GEO

Effect of potassium-depleted diet on kidney

Project description:Libraries obtained from microdissected kidney outer medullary collecting duct from C57Bl6/J mice control or after 3 days potassium-depleted diet. Keywords: other

2004-05-30 | E-GEOD-1445 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data