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Candida albicans proteomics_planktonic culture without treatment

ABSTRACT: LC-MS/MS approach was utilized to identify qualitative changes in protein expressed by planktonic and biofilm cells of C. albicans.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Mun Fai Loke

LAB HEAD: Loke Mun Fai

PROVIDER: PXD007831 | Pride | 2021-02-25

REPOSITORIES: Pride

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			Action	DRS
	300a.mgf	Mgf
	300a.mzxml	Mzxml
	301a.mgf	Mgf
	301a.mzxml	Mzxml
	302a.mgf	Mgf

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Publications

LC-MS analysis reveals biological and metabolic processes essential for Candida albicans biofilm growth.

Munusamy Komathy K Loke Mun Fai MF Vadivelu Jamuna J Tay Sun Tee ST

Microbial pathogenesis 20201114

Candidiasis is the most common fungal infection associated with high morbidity and mortality among immunocompromised patients. The ability to form biofilm is essential for Candida albicans pathogenesis and drug resistance. In this study, the planktonic cell and biofilm proteomes of C. albicans SC5314 strain analyzed using Liquid Chromatography-Mass Spectrometry (LC-MS) were compared. In total, 280 and 449 proteins are annotated from the planktonic cell and biofilm proteomes, respectively. The bi ...[more]

PMID: 33202254

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Candida albicans proteomics_Biofilm without treatment

Project description:The LC-MS/MS approach was utilized to identify qualitative changes in protein expressed by planktonic and biofilm cells of C. albicans and with Aureobasidin treatment.

2021-02-25 | PXD007830 | Pride

Candida albicans proteomics_planktonic with Aureobasidin treatment

Project description:LC-MS/MS approach was utilized to identify qualitative changes in protein expressed by planktonic and biofilm cells of C. albicans.

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Candida albicans proyeomics_Planktonic DMSO control

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Candida albicans proteomics_biofilm with Aureobasidin treatment

Project description:LC-MS/MS approach was utilized to identify qualitative changes in protein expressed by planktonic and biofilm cells of C. albicans.

2021-11-25 | PXD007834 | Pride

Candida albicans proteomics_biofilm DMSO control

Project description:LC-MS/MS approach was utilized to identify qualitative changes in protein expressed by planktonic and biofilm cells of C. albicans.

2021-11-25 | PXD007832 | Pride

Increased mutability of staphylococci in biofilms as a consequence of oxidative stress

Project description:In this work we have demonstrated increased mutability of Staphylococcus aureus and S. epidermidis in biofilms and have explored the mechanisms underlying the enhanced mutability. A novel static biofilm model, utilising cellulose filter disks, was developed to support the formation of mature biofilms with sufficiently high cell densities to permit determination of mutation frequencies. The mutability of biofilm cultures increased up to 60 fold and 4 fold for S. aureus and S. epidermidis, respectively, compared with planktonic cultures. Incorporation of antioxidants into S. aureus biofilms reduced mutation frequencies, indicating that increased oxidative stress underlies increased mutability in the biofilm. Transcriptional profiling revealed upregulation of the superoxide dismutase gene, sodA, in early biofilm cultures, also suggesting enhanced oxidative stress in these cultures. However, loss of the genes encoding superoxide dismutases or peroxidases did not specifically exacerabate biofilm mutability. In S. aureus SH1000, hydrogen peroxide was found to contribute to biofilm mutability. Three growth conditions (18 hr planktonic growth, 48 hr biofilm growth and 144 biofilm growth) of which there are three biological replicates of each

2012-03-01 | E-GEOD-35837 | biostudies-arrayexpress

RNA-Seq analysis of transcriptomes of B. amyloliquefaciens FZB42 in biofilm formation and planktonic cells.

Project description:This experiment compares the transcriptomes of B. amyloliquefaciens FZB42 cells in biofilm formation and in planktonic cells focusing on transcripts that were most affected in their transcription. The aim of the experiment was determination of the highest expressed genes in both cultures using RPKM values and comparison of relative transcription rates using DeSeq analysis.

2017-03-16 | E-MTAB-4362 | biostudies-arrayexpress

Transcriptional Response of the thermoacidophilic Archaeon Sulfolobus acidocaldarius to solvent stress exemplified by 1-butanol exposure

Project description:To study the cellular global response of the thermoacidophilic Archaeon Sulfolobus acidocaldarius upon solvent exposure we performed transcriptome comparing the response of planktonic and biofilm cells in absence and presence 1-butanol. S. acidocaldarius was grown in Petri dishes (static incubation) in the absence and presence of 0.5% and 1% (v/v) 1-butanol at 76°C for four days. Planktonic and biofilm cells were harvested and the transcriptional response towards 1-butanol was analysed via RNA-seq. In detail:Biofilm and planktonic cell samples (supernatant after static incubation) were generated in Petri dishes (92 x 16 mm, Polystyrene, without cams, Sarstedt) as described before. Cells grown under six different conditions, including Biofilm-Control (BF0), Biofilm-0.5% (v/v) 1-Butanol (BF05), Biofilm-1% (v/v) 1-Butanol (BF1), Planktonic-Control (PL0), Planktonic-0.5% (v/v) 1-Butanol (PL05), Planktonic-1% (v/v) 1-Butanol (PL1), were seperated, harvested by centrifugation (10 min, 5,000 x g, 4 °C) and immediately frozen at -70 °C. Isolation of cells was performed in triplicates with ten technical replicas each. Samples were pooled to obtain sufficient cell mass for further processing. RNA was isolated using Trizol (Thermo Fisher Scientific). In accordance, sequencing libraries were prepared via Illumina TruSeq stranded mRNA using Ribozero for RNA depletion. Sequencing was performed on a MiSeq instrument (Illumina, San Diego, California, USA) using v3 chemistry with a read length of 2x76 nt.

2021-02-20 | E-MTAB-10093 | biostudies-arrayexpress

Transcriptome of A. pleuropneumoniae biofilms cultured under different growth conditions

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-05-20 | E-GEOD-43824 | biostudies-arrayexpress

E coli MG1655 biofilm and planktonic gene expression

Project description:Bacteria growing as surface-adherent biofilms are better able to withstand chemical and physical stresses than their unattached, planktonic counterparts. Using transcriptional profiling and quantitative PCR, we observed a previously uncharacterized gene, yjfO, to be upregulated during Escherichia coli MG1655 biofilm growth in a chemostat on serine-limited defined medium. A yjfO mutant, developed through targeted insertion mutagenesis, and a yjfO-complemented strain, were obtained for further characterization. While bacterial surface colonization levels (CFU/cm2) were similar in all three strains, the mutant strain exhibited reduced microcolony formation when observed in flow cells, and greatly enhanced flagellar motility on soft (0.3%) agar. Complementation of yjfO restored microcolony formation and flagellar motility to wild type levels. Cell surface hydrophobicity and twitching motility were unaffected by the presence or absence of yjfO. In contrast to the parent strain, biofilms from the mutant strain were less able to resist acid and peroxide stresses. yjfO had no significant effect on E. coli biofilm susceptibility to alkali or heat stress. Planktonic cultures from all three strains showed similar responses to these stresses. Regardless of the presence of yjfO, planktonic E. coli withstood alkali stress better than biofilm populations. Complementation of yjfO restored viability following exposure to peroxide stress, but did not restore acid resistance. Based on its influence on biofilm formation, stress response, and effects on motility, we propose renaming the uncharacterized gene, yjfO, as bsmA (biofilm stress and motility). Transcriptional profiling of duplicate biofilm and planktonic cultures of E. coli MG1655 grown in serine-limited MOPS minimal media.

2010-06-21 | E-GEOD-18362 | biostudies-arrayexpress

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