Proteomics

Dataset Information

0

Comparative analysis of mRNA degradation and protein degradation in 68 pairs of adjacent prostate tissue samples indicates high stability of proteins


ABSTRACT: Here we investigated the degradation of mRNA and protein in 68 pairs of adjacent prostate tissue samples using RNA-seq and pressure cycling technology (PCT) coupled with SWATH mass spectrometry and developed a score, the Proteome Integrity Number (PIN), to quantify the extent of protein degradation in the samples.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Prostate Adenocarcinoma Cell, Epithelial Cell

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Wenguang Shao  

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD007841 | Pride | 2019-06-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ConvertTSVToTraML.TraML Other
feature_alignment_68samples.tsv Tabular
guot_L140220sw_01B.wiff Wiff
guot_L140220sw_01B.wiff.scan Wiff
guot_L140220sw_02.wiff Wiff
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Publications


Deterioration of biomolecules in clinical tissues is an inevitable pre-analytical process, which affects molecular measurements and thus potentially confounds conclusions from cohort analyses. Here, we investigate the degradation of mRNA and protein in 68 pairs of adjacent prostate tissue samples using RNA-Seq and SWATH mass spectrometry, respectively. To objectively quantify the extent of protein degradation, we develop a numerical score, the Proteome Integrity Number (PIN), that faithfully mea  ...[more]

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