Dataset Information

Tef (Eragrostis tef) iTRAQ-labelled protein fractions

ABSTRACT: The orphan crop, Eragrostis tef, was subjected to controlled drought conditions to observe the physiological parameters and proteins changing in response to dehydration stress. Physiological measurements involving electrolyte leakage, chlorophyll fluorescence and ultra-structural analysis showed tef plants to tolerate water loss to 50% RWC before adverse effects in leaf tissues were observed. Proteomic analysis using iTRAQ mass spectrometry and appropriate database searching enabled the detection of 5727 proteins, of which 211 proteins, including a number of spliced variants, were found to be differentially regulated with imposed stress conditions. Validation of the stress-related proteins, fructose-bisphosphate aldolase (FBA), glutamine synthetase (GLN) and the protective antioxidant proteins, monodehydroascorbate reductase (MDHAR) and peroxidase (POX), using immunodetection and enzymatic assay confirmed protein presence according to iTRAQ findings and showed increased protein abundance levels and enzymatic activity in response to water-deficit. GO-term enrichment and analysis revealed terms involved in biotic and abiotic stress response, signaling, transport, cellular homeostasis and pentose metabolic processes, to be enriched in tef up-regulated proteins, while terms linked to ROS-producing processes under water-deficit, such as photosynthesis and associated light harvesting reactions, manganese transport and homeostasis, the synthesis of sugars and cell wall catabolism and modification, to be enriched in tef down-regulated proteins.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Hordeum Vulgare (barley) Oryza Sativa (rice) Zea Mays (maize)

TISSUE(S): Leaf

SUBMITTER: Rizqah Kamies

LAB HEAD: Dr Suhail Rafudeen

PROVIDER: PXD007907 | Pride | 2017-11-21

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	ProteinSummary.txt	Txt
	Rawdata.d.zip	Other
	Refinedandprocessedlistoftefproteins-peptidesforprotviz.xlsx	Xlsx
	TeffilelistdescriptionREADME	Other
	protein-peptides_quantdata.xlsx	Xlsx

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Publications

A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef.

Kamies Rizqah R Farrant Jill M JM Tadele Zerihun Z Cannarozzi Gina G Rafudeen Mohammed Suhail MS

Proteomes 20171115 4

The orphan crop, <i>Eragrostis tef</i>, was subjected to controlled drought conditions to observe the physiological parameters and proteins changing in response to dehydration stress. Physiological measurements involving electrolyte leakage, chlorophyll fluorescence and ultra-structural analysis showed tef plants tolerated water loss to 50% relative water content (RWC) before adverse effects in leaf tissues were observed. Proteomic analysis using isobaric tag for relative and absolute quantifica ...[more]

PMID: 29140297

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