Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: Victoria Harman
LAB HEAD: Robert Jeffrey Beynon
PROVIDER: PXD007949 | Pride | 2017-10-27
REPOSITORIES: Pride
Items per page: 1 - 5 of 14 |
Molecular & cellular proteomics : MCP 20171020 12
A major challenge in proteomics is the absolute accurate quantification of large numbers of proteins. QconCATs, artificial proteins that are concatenations of multiple standard peptides, are well established as an efficient means to generate standards for proteome quantification. Previously, QconCATs have been expressed in bacteria, but we now describe QconCAT expression in a robust, cell-free system. The new expression approach rescues QconCATs that previously were unable to be expressed in bac ...[more]