Proteomics

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Venom proteomics of Naja naja of western India origin

ABSTRACT: Bites by the Indian spectacled cobra (Naja naja) are widely reported across the Indian subcontinent, with an associated high rate of mortality and morbidity. In western India (WI), the numbers of reported incidents of cobra envenomation are significantly higher than the other snake bites. In this study the venom proteome of WINn was deciphered for the first time using tandem mass spectroscopy analysis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Naja Naja

TISSUE(S): Saliva

SUBMITTER: Abhishek Chanda  

LAB HEAD: Dr. A. K. Mukherjee

PROVIDER: PXD008011 | Pride | 2019-01-07

REPOSITORIES: Pride

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Ashis_1.RAW Raw
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Publications

Proteomic analysis and antivenomics study of Western India <i>Naja naja</i> venom: correlation between venom composition and clinical manifestations of cobra bite in this region.

Chanda Abhishek A   Kalita Bhargab B   Patra Aparup A   Senevirathne Wanigasingha D Sandani T WDST   Mukherjee Ashis K AK  

Expert review of proteomics 20181224 2

<b>Background</b>: Snakebite is a severe problem in the tropical countries including Indian subcontinent. Premier cases of cobra bites are being reported from western India (WI). <b>Research design and methods</b>: The proteome of WI <i>N. naja</i> venom (NnV) was deciphered by high resolution mass spectrometry analysis of venom, further fractionated by gel filtration (GF) or RP-HPLC followed by SDS-PAGE and then tandem mass spectrometric analysis of protein bands. The efficacy of commercial pol  ...[more]

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