Proteomics

Dataset Information

0

Cadmium specific proteomic responses of Pseudomonas aeruginosa san ai


ABSTRACT: Pseudomonas aeruginosa san ai resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to sub-lethal concentration of cadmium (0.9 mM) adsorbed 75% of added cadmium after 48 hours, while remaining 25% in culture supernatant was adsorbed by exopollysaccaryde, implying a large biosorption potential of leaving biomass and its exopollysaccaryde. Inner cell response on the protein level was investigated by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins in complex protein fractions obtained by size exclusion chromatography used to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 59 proteins were observed as up-regulated in cadmium- amended culture. Almost a third of the total number of up-regulated were metalloproteins. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in cell energy metabolism, amino acid metabolism, cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium- amended culture versus control was found. Our results signify that P. aeruginosa san ai has a great potential for application in bioremediation of cadmium polluted sites.

INSTRUMENT(S): amaZon Speed

ORGANISM(S): Pseudomonas Aeruginosa Atcc 15442

SUBMITTER: Ivanka Karadzic  

LAB HEAD: Ivanka Karadzic

PROVIDER: PXD008020 | Pride | 2022-05-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Fraction_I1.csv Csv
Fraction_I1.d.zip Other
Fraction_I1.mgf Mgf
Fraction_I2.csv Csv
Fraction_I2.d.zip Other
Items per page:
1 - 5 of 21
altmetric image

Publications


<i>Pseudomonas aeruginosa</i> san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of <i>P. aeruginosa</i> san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tan  ...[more]

Similar Datasets

2021-06-25 | PXD021643 | Pride
2015-11-01 | E-GEOD-73744 | biostudies-arrayexpress
2022-11-27 | PXD033853 | Pride
2011-01-25 | E-GEOD-26828 | biostudies-arrayexpress
2012-03-25 | E-GEOD-36627 | biostudies-arrayexpress
2008-06-16 | E-GEOD-9480 | biostudies-arrayexpress
2009-03-07 | E-GEOD-8013 | biostudies-arrayexpress
2005-12-01 | E-MEXP-416 | biostudies-arrayexpress
2008-08-21 | E-GEOD-12373 | biostudies-arrayexpress
2010-05-25 | E-GEOD-9221 | biostudies-arrayexpress