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Dynamics of venom composition across a complex life cycle

ABSTRACT: Nematostella vectensis is a venomous organism with a complex life cycle. We harness different approaches to study venom composition dynamics along its life cycle. Our methods include but not limited to transcriptomica and proteomics. Here we perform semi quantitative analysis of Nematostella proteom at 4 developmental stages to compare abundance of different toxins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Nematostella Vectensis

TISSUE(S): Embryo, Egg, Whole Body

SUBMITTER: Maria Sachkova

LAB HEAD: Yehu Moran

PROVIDER: PXD008218 | Pride | 2018-02-14

REPOSITORIES: Pride

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Publications

Dynamics of venom composition across a complex life cycle.

Columbus-Shenkar Yaara Y YY Sachkova Maria Y MY Macrander Jason J Fridrich Arie A Modepalli Vengamanaidu V Reitzel Adam M AM Sunagar Kartik K Moran Yehu Y

eLife 20180209

Little is known about venom in young developmental stages of animals. The appearance of toxins and stinging cells during early embryonic stages in the sea anemone <i>Nematostella vectensis</i> suggests that venom is already expressed in eggs and larvae of this species. Here, we harness transcriptomic, biochemical and transgenic tools to study venom production dynamics in <i>Nematostella</i>. We find that venom composition and arsenal of toxin-producing cells change dramatically between developme ...[more]

PMID: 29424690

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Project description:Comparative interactome analysis of Nematostella vectensis Hsp70 isoforms reveals unique clientome remodeling upon heat stress. Three isoforms of nvHSP70 (A, B, and D) were IP'd from either Heat Shock conditions (HS) or non-Heat Shock (UN) and their interactomes were compared. Mass Spec was run on a Q-Exactive (Orbitrap).

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Project description:Bilaterian animals differ from other metazoans in their apparent bilateral symmetry and the development of a third germ layer. Both might have facilitated the evolution of the diverse and complex bilaterian body plans. The first cnidarian genome sequence revealed that despite their morphological simplicity, this sister group to all bilaterians shares an immense genomic complexity with vertebrates. This suggested that it might have been the complexity of gene regulation which increased during the evolution of bilaterians. We compared the gene regulatory landscape of cnidarians and bilaterians. To this end we generated the first genome-wide prediction of gene regulatory elements and profiled five epigenetic marks in a non-bilaterian animal, the cnidarian Nematostella vectensis. We found that the location of chromatin modifications relative to genes and distal enhancers is conserved among eumetazoans. Surprisingly, the genomic landscape of gene regulatory elements is highly similar between Nematostella and bilaterian model organisms. This suggests that complex regulation of developmental gene expression evolved in eumetazoans without a major increase in complexity in bilaterians. ChIP-seq of p300, RNA Pol2, and five histone modifications in Nematostella vectensis.

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