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ALKBH7, a mitochondrial member of AlkB family, binds histones in vitro, part 3

ABSTRACT: Human genome encodes 9 AlkB family members, one of which is ALKBH7. In this study, we found recombinant ALKBH7 could bind bulk histones in GST pull-down and attenuate the signal of H4K20me2 in demethylation assays. With mass spectrometry, we also identified several potential ALKBH7-interacting proteins, such as NDUFS2, MRPS7 etc.; and found the expression of NDUFA4 and COX6C was down-regulated upon knockdown of ALKBH7. With these results, we can obtain a novel sight into the function of this protein

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Wei Ge

LAB HEAD: Wei Ge

PROVIDER: PXD008320 | Pride | 2020-05-26

REPOSITORIES: Pride

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			Action	DRS
	01-05.msf	Msf
	01-05.pep.xml	Pepxml
	01-05.xlsx	Xlsx
	010304.raw	Raw
	02.raw	Raw

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Publications

The interactome and proteomic responses of ALKBH7 in cell lines by in-depth proteomics analysis.

Meng Shu S Zhan Shaohua S Dou Wanchen W Ge Wei W

Proteome science 20191229

<h4>Background</h4>ALKBH7 is a mitochondrial protein, involved in programmed necrosis, fatty acid metabolism, cell cycle regulation, and prostate cancer disease. However, the exact roles of ALKBH7 and the underlying molecular mechanisms remain mysterious. Thus, investigations of the interactome and proteomic responses of ALKBH7 in cell lines using proteomics strategies are urgently required.<h4>Methods</h4>In the present study, we investigated the interactome of ALKBH7 in mitochondria through im ...[more]

PMID: 31889914

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ALKBH7, a mitochondrial member of AlkB family, binds histones in vitro, part 2

Project description:Human genome encodes 9 AlkB family members, one of which is ALKBH7. In this study, we found recombinant ALKBH7 could bind bulk histones in GST pull-down and attenuate the signal of H4K20me2 in demethylation assays. With mass spectrometry, we also identified several potential ALKBH7-interacting proteins, such as NDUFS2, MRPS7 etc.; and found the expression of NDUFA4 and COX6C was down-regulated upon knockdown of ALKBH7. With these results, we can obtain a novel sight into the function of this protein.

2020-05-26 | PXD008319 | Pride

ALKBH7, a mitochondrial member of AlkB family, binds histones in vitro, part 1

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Reduced expression of mitochondrial complex I subunit Ndufs2 does not impact healthspan in mice

Project description:Aging in mammals leads to reduction in genes encoding the 45-subunit mitochondrial electron transport chain (ETC) complex I. It has been hypothesized that normal aging and age-related diseases such as Parkinson’s disease are in part due to modest decrease in expression of mitochondrial complex I subunits. By contrast, diminishing expression of mitochondrial complex I genes in lower organisms increases lifespan. Furthermore, metformin, a putative complex I inhibitor increases healthspan in mice and humans. In the present study, we investigated whether loss of one allele of Ndufs2, the catalytic subunit of mitochondrial complex I, impacts healthspan and lifespan in mice. Our results indicate that Ndufs2 hemizygous mice (Ndufs2+/-) show no overt impairment in motor function, learning, tissue histology, organismal metabolism, or sensitivity to metformin in a C57B6/J background. However, there are detectable changes at the level of gene expression in individual cell types and tissues due to loss of one allele of Ndufs2. Our data indicate that modest decline in expression of mitochondrial complex I subunit Ndufs2 is not detrimental to healthspan.

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A Human Induced Pluripotent Stem Cell Array-Based Genome Wide Association Study Identifies Virus Susceptibility Locus and Variants

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Plant microRNAs in WT and SIDT1 KO mice liver

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| PRJNA257233 | ENA