Proteomics

Dataset Information

0

Repetitive somatic embryogenesis induced cytological and proteomic changes in propagated embryogenic lines of Pseudotsuga menziesii [Mirb.]

ABSTRACT: Objectives: to characterize and to better understand changes at the cellular and molecular levels, in embryogenic lines in Douglas-fir obtained after repetitive somatic embryogenesis, that could explain improvement of their embryogenic potential : to primary lines, secondary ones showed an increase of their embryogenic potential; then tertiary lines showed again an improved embryogenic potential compared to second lines. Interestingly, the proteomic analysis further revealed different sets of significant proteins suggesting that each cycle of repetitive somatic embryogenesis is promoting substantial genome-wide rearrangement of the gene expression pattern.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pseudotsuga Menziesii

SUBMITTER: Stephane Claverol  

LAB HEAD: Marc Bonneu

PROVIDER: PXD008347 | Pride | 2018-08-15

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
ct150915_09.msf Msf
ct150915_09.raw Raw
ct150915_10.msf Msf
ct150915_10.raw Raw
ct150915_11.msf Msf
Items per page:
1 - 5 of 48
altmetric image

Publications

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.].

Gautier Florian F   Eliášová Kateřina K   Leplé Jean-Charles JC   Vondráková Zuzana Z   Lomenech Anne-Marie AM   Le Metté Claire C   Label Philippe P   Costa Guy G   Trontin Jean-François JF   Teyssier Caroline C   Lelu-Walter Marie-Anne MA  

BMC plant biology 20180810 1

<h4>Background</h4>To explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2<sup>ry</sup>) and tertiary (3<sup>ry</sup>) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2<sup>ry</sup> lines exhibited significantly higher embryogenic potential (SE yields) than the 1<sup>ry</sup> lines initiated from zygotic embryos (SD4, 2155 vs 477; TD17, 240 vs 29 g<sup>- 1</sup> f.w.). Moreo  ...[more]

Similar Datasets

Proteomics Cytological, biochemical and molecular events related to the embryogenic state in Douglas-fir
2019-05-17 | PXD011176 | Pride
Transcriptomics embryogenesis ws-somatic embryogenesis
2013-04-11 | E-GEOD-45940 | biostudies-arrayexpress
Transcriptomics embryogenesis col0-somatic embryogenesis
2012-05-31 | E-GEOD-30425 | biostudies-arrayexpress
Transcriptomics embryogenesis ws-somatic embryogenesis
2012-05-31 | E-GEOD-30383 | biostudies-arrayexpress
Proteomics Secretome analysis revealed amylases and peroxidases as markers of the early stages of somatic embryogenesis in Pinus nigra
2023-07-28 | PXD040247 | Pride
Transcriptomics embryogenesis col0-somatic embryogenesis
2012-06-01 | GSE30425 | GEO
Transcriptomics embryogenesis ws-somatic embryogenesis
2012-06-01 | GSE30383 | GEO
Proteomics Embryogenic competence acquisition in sugarcane callus is associated with differential H+ pump abundance and activity
2018-06-26 | PXD009858 | Pride
Proteomics A proteomic analysis of the surface attachment in Pseudomonas aeruginosa early as 20 minutes incubation.
2017-07-11 | PXD002942 | Pride
Proteomics Platelet proteomics profiling in transfusion adverse events, part 2
2019-02-13 | PXD003510 | Pride