Proteomics

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Shotgun proteomic profiling of head kidney of rainbow trout Oncorhynchus mykiss

ABSTRACT: The objective of this study was to identify and quantify proteomic profiles of head kidney of rainbow trout Oncorhynchus mykiss. Specific pathogen free rainbow trout (mean length 15 ± 1 cm) were maintained in recirculating de-chlorinated water at 19±1 °C. Prior to the experiment, fish were distributed between 9 aquaria, 18 fish per aquarium. The test groups were infected by immersion of Yersinia ruckeri strains: CSF007-82 (biotype 1) and 7959-11 (biotype 2). The control group was immersed similar with sterile broth medium. There were 3 aquaria per each group (CSF007-82-infected, 7959-11-infected and control). Nine fish from infected and control fish groups were anaesthetized with MS-222 at 3, 9 and 28 days post exposure and sampled aseptically. Each head kidney was washed three times with sterile phosphate-buffered saline containing a cocktail of mammalian protease inhibitors. Head kidney samples were snap-frozen in liquid nitrogen and stored at –80 °C.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri)

TISSUE(S): Kidney

SUBMITTER: Gokhlesh Kumar  

LAB HEAD: Dr. Gokhlesh Kumar

PROVIDER: PXD008473 | Pride | 2018-08-14

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
IDA_FASP_Kidney_7959_D28_P1.mgf Mgf
IDA_FASP_Kidney_7959_D28_P1.wiff Wiff
IDA_FASP_Kidney_7959_D28_P1.wiff.scan Wiff
IDA_FASP_Kidney_7959_D28_P1.xml Xml
IDA_FASP_Kidney_7959_D28_P2.mgf Mgf
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Publications

Proteome Profiles of Head Kidney and Spleen of Rainbow Trout (Oncorhynchus Mykiss).

Kumar Gokhlesh G   Hummel Karin K   Razzazi-Fazeli Ebrahim E   El-Matbouli Mansour M  

Proteomics 20180816 17

The head kidney and spleen are major lymphoid organs of the teleost fish. The authors identify proteome profiles of head kidney and spleen of rainbow trout (Oncorhynchus mykiss) using a shotgun proteomic approach. Gene ontology annotation of proteins is predicted using bioinformatic tools. This study represents detailed proteome profiles of head kidney and spleen of rainbow trout, with a total of 3241 and 2542 proteins identified, respectively. It is found that lymphoid organs are equipped with  ...[more]

PMID: 30094954

Publication: 1/2

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