Dataset Information

Shotgun proteomic profiling of intestinal mucosa of rainbow trout Oncorhynchus mykiss

ABSTRACT: The objective of this study was to identify and quantify proteomic profiles of intestine of rainbow trout (Oncorhynchus mykiss). Specific pathogen free rainbow trout (mean length 15 ± 1 cm) were maintained in recirculating de-chlorinated water at 19±1 °C. Prior to the experiment, fish were distributed between aquaria. The test groups were infected by immersion of Yersinia ruckeri CSF007-82 (biotype 1) and 7959-11 (biotype 2) strains. The control group was immersed similar with sterile broth medium. Fish were anaesthetized and sampled aseptically at different time points. Each intestine was washed three times with sterile phosphate-buffered saline containing a cocktail of mammalian protease inhibitors. Intestinal mucosa was scraped with a sterile large scalpel blade. Intestinal samples were snap-frozen in liquid nitrogen and stored at –80 °C.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri)

TISSUE(S): Small Intestine Mucosa

SUBMITTER: Gokhlesh Kumar

LAB HEAD: Dr. Gokhlesh Kumar

PROVIDER: PXD011087 | Pride | 2019-11-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	IDA_FASP_Intestine_7959_D3_P1.mgf	Mgf
	IDA_FASP_Intestine_7959_D3_P1.wiff	Wiff
	IDA_FASP_Intestine_7959_D3_P1.wiff.scan	Wiff
	IDA_FASP_Intestine_7959_D3_P1.xml	Xml
	IDA_FASP_Intestine_7959_D3_P2.mgf	Mgf

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Publications

Modulation of posterior intestinal mucosal proteome in rainbow trout (Oncorhynchus mykiss) after Yersinia ruckeri infection.

Kumar Gokhlesh G Hummel Karin K Razzazi-Fazeli Ebrahim E El-Matbouli Mansour M

Veterinary research 20190717 1

Yersinia ruckeri is the causative agent of enteric redmouth disease in salmonids. In fish, the intestine represents an important site of nutrient uptake, host-pathogen interactions, and defense. The posterior intestine can be inflamed, reddened, and filled with an opaque, yellowish fluid during Y. ruckeri infection. Herein, we report an investigation on the proteome alteration in the posterior intestinal mucosa of rainbow trout (Oncorhynchus mykiss) after exposure to Y. ruckeri. The intestinal m ...[more]

PMID: 31315687

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