Proteomics

Dataset Information

0

Lamin A acetylation shapes nuclear architecture and safeguards genomic integrity

ABSTRACT: The nuclear lamina constitutes more than a structural scaffold for the nucleus and plays a crucial role in protection of genomic integrity. Here we report that the loss of the lysine acetyl-transferase (KAT) MOF leads to nuclear architecture defects during interphase including micronuclei formation. We identify Lamin A/C, a major component of the nuclear lamina, to be an acetylation target of MOF. A point mutation in Lamin A phenocopies nuclear morphology defects observed upon Mof-deletion. Through single cell DNA sequencing, we reveal that either loss of Mof or Lamin A mutation result in extensive genomic instability, including chromothripsis. Our work establishes MOF-dependent Lamin acetylation as a key regulator of nuclear architecture maintenance in mammals.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Fibroblast, Embryonic Stem Cell

SUBMITTER: Witold Szymanski  

LAB HEAD: Gerhard Mittler

PROVIDER: PXD008539 | Pride | 2019-08-16

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
2015_01_AcIP_IN_rev__01.raw Raw
2015_01_AcIP_fwd_2_01.raw Raw
2015_01_AcIP_fwd_2_03.raw Raw
2015_01_AcIP_rev_1_01.raw Raw
2015_01_AcIP_rev_1_02.raw Raw
Items per page:
1 - 5 of 269
altmetric image

Publications

While nuclear lamina abnormalities are hallmarks of human diseases, their interplay with epigenetic regulators and precise epigenetic landscape remain poorly understood. Here, we show that loss of the lysine acetyltransferase MOF or its associated NSL-complex members KANSL2 or KANSL3 leads to a stochastic accumulation of nuclear abnormalities with genomic instability patterns including chromothripsis. SILAC-based MOF and KANSL2 acetylomes identified lamin A/C as an acetylation target of MOF. HDA  ...[more]

Similar Datasets

Proteomics Kansl2 dependent acetylome study in primary Kansl2 (fl/fl) murine embryonic fibroblasts
2023-01-05 | PXD038521 | Pride
Transcriptomics The activation potential of MOF is constrained for dosage compensation
2010-06-26 | E-GEOD-20695 | biostudies-arrayexpress
Transcriptomics A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated polypeptide 2alpha [gene expression]
2016-01-19 | E-GEOD-70148 | biostudies-arrayexpress
Transcriptomics MOF acetyl transferase regulates transcription and respiration in mitochondria
2016-10-21 | E-GEOD-77784 | biostudies-arrayexpress
Transcriptomics MOF acetyl transferase regulates transcription and respiration in mitochondria
2016-10-21 | GSE77784 | GEO
Proteomics Nuclear lamin A/C phosphorylation by loss of Androgen Receptor is a global determinant of cancer-associated fibroblast activation
2024-08-12 | PXD042059 | Pride
Transcriptomics The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset
2011-10-28 | E-GEOD-30991 | biostudies-arrayexpress
Transcriptomics Spatially restricted position-dependent implications of lamin A promoter occupancy on gene activity (ChIPs and MeDips)
2013-07-14 | E-GEOD-42522 | biostudies-arrayexpress
Proteomics Lamin B1 acetylation slows the G1 to S cell cycle transition through inhibition of DNA repair
2021-01-08 | PXD022974 | Pride
Transcriptomics ChIP-chip profiles of MLE, MSL3 and MOF in Drosophila S2 cells
2012-12-11 | E-GEOD-37863 | biostudies-arrayexpress