Proteomics

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BCL11B_AP-MS - The N-terminal CCHC zinc finger motif mediates homodimerization of the transcription factor BCL11B

ABSTRACT: This study investigates whether the N-terminal CCHC zinc finger motif is necessary and sufficient for the formation of BCL11B dimers, using fluorescence microscopy and Förster resonance energy transfer-assisted fluorescence-activated cell sorting (FACSFRET). The role of the CCHC motif in homodimer assembly was further investigated using affinity purification followed by mass spectrometry (AP-MS). Functional studies investigated the role of dimerization in the induction of cell cycle arrest and protection against DNA damage-induced apoptosis.

INSTRUMENT(S): TSQ Vantage, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell

SUBMITTER: Praveen Sappa  

LAB HEAD: Christian Schmidt

PROVIDER: PXD008606 | Pride | 2018-01-12

REPOSITORIES: Pride

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The N-Terminal CCHC Zinc Finger Motif Mediates Homodimerization of Transcription Factor BCL11B.

Grabarczyk Piotr P   Winkler Passorn P   Delin Martin M   Sappa Praveen K PK   Bekeschus Sander S   Hildebrandt Petra P   Przybylski Grzegorz K GK   Völker Uwe U   Hammer Elke E   Schmidt Christian A CA  

Molecular and cellular biology 20180212 5

The <i>BCL11B</i> gene encodes a Krüppel-like, sequence-specific zinc finger (ZF) transcription factor that acts as either a repressor or an activator, depending on its posttranslational modifications. The importance of <i>BCL11B</i> in numerous biological processes in multiple organs has been well established in mouse knockout models. The phenotype of the first <i>de novo</i> monoallelic germ line missense mutation in the <i>BCL11B</i> gene (encoding N441K) strongly implies that the mutant prot  ...[more]

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