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The PRC1 and PRC2 proximal interactome

ABSTRACT: Polycomb Repressive Complexes PRC1 and PRC2 play a crucial role in silencing lineage-specific genes during early embryogenesis. To provide new insights in polycomb biology, we profiled the proximal interactome (proxeome) of the catalytic subunits RNF2 (PRC1) and EZH2 (PRC2) in mouse embryonic stem cells (mESCs). This revealed >100 proteins proximal to PRC2 and PRC1, which mainly comprise transcription factors, transcriptional regulators and RNA binding proteins. Interestingly, the EZH2 proxeome included both PRC complexes, while the RNF2 proxeome only identified PRC1 subunits. More than half of the PRC2 proximal proteins are shared with PRC1, revealing the molecular constitution of polycomb chromatin domains. We identified several pluripotency-associated transcription factors, including NANOG, for which we confirmed genomic co-localisation with PRC2. Upon PRC2 disruption, NANOG redistributes to specific sites containing its DNA binding motif. Finally, we compared PRC2 proximal interactomes between naïve mESCs, serum-cultured mESCs and embryoid bodies, altogether providing a comprehensive resource in different cellular contexts that may help to further decipher Polycomb biology.

INSTRUMENT(S): Orbitrap Fusion ETD, Q Exactive HF-X, Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Dick Zijlmans

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD047569 | Pride | 2025-02-25

REPOSITORIES: pride

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			Action	DRS
	20191122_Fusion1_SA_DZ013_ET1_FT_1.raw	Raw
	20191122_Fusion1_SA_DZ013_ET1_FT_2.raw	Raw
	20191122_Fusion1_SA_DZ013_ET1_FT_3.raw	Raw
	20191122_Fusion1_SA_DZ013_ET1_pH2_1.raw	Raw
	20191122_Fusion1_SA_DZ013_ET1_pH2_2.raw	Raw

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The PRC1 and PRC2 proximal interactome

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