Dataset Information

The Bordetella pertussis and Bordetella bronchiseptica filamentous hemagglutinins are processed at different sites

ABSTRACT: Mapping of the C-terminal residues of FHA from Bordetellae utilizing differential 16O/18O labelling

INSTRUMENT(S): Bruker Daltonics solarix series

ORGANISM(S): Bordetella Pertussis Bordetella Bronchiseptica Rb50

TISSUE(S): Cell Culture

SUBMITTER: David Jurnečka

LAB HEAD: Peter Sebo

PROVIDER: PXD008664 | Pride | 2018-10-22

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 52

Publications

Bordetella pertussis and Bordetella bronchiseptica filamentous hemagglutinins are processed at different sites.

Jurnecka David D Man Petr P Sebo Peter P Bumba Ladislav L

FEBS open bio 20180620 8

Filamentous hemagglutinin (FHA) mediates adherence and plays an important role in lower respiratory tract infections by pathogenic Bordetellae. The mature FHA proteins of B. pertussis (Bp-FHA) and the B. bronchiseptica (Bb-FHA) are generated by processing of the respective FhaB precursors by the autotransporter subtilisin-type protease SphB1. We have used bottom-up proteomics with differential 16O/18O labeling and show that despite high-sequence conserv ...[more]

PMID: 30087831

Similar Datasets

Project description:Mycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly impact on human and animal health worldwide. Mycobacterial life cycle is complex and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Eurasian wild boar (Sus scrofa) are natural reservoir hosts for MTBC and a model for mycobacterial infection and tuberculosis (TB). In the wild boar TB model, mycobacterial infection affects the expression of innate and adaptive immune response genes in mandibular lymph nodes and oropharyngeal tonsils and biomarkers have been proposed as correlates with resistance to natural infection. However, the mechanisms used by mycobacteria to manipulate host immune response are not fully characterized. Our hypothesis is that the immune system proteins under-represented in infected animals when compared to uninfected controls are used by mycobacteria to guarantee pathogen infection and transmission. To address this hypothesis, a comparative proteomics approach was used to compare host response between uninfected (TB-) and M. bovis-infected young (TB+) and adult animals with different infection status [TB lesions localized in the head (TB+) or affecting multiple organs (TB++)]. The results identified host immune system proteins that play an important role in host response to mycobacteria. Calcium binding protein A9, Heme peroxidase, Lactotransferrin, Cathelicidin and Peptidoglycan-recognition protein were under-represented in TB+ animals when compared to uninfected TB- controls but protein levels increased as infection progressed in TB++ animals when compared to TB- and/or TB+ adult wild boar. MHCI was the only protein over-represented in TB+ adult wild boar when compared to uninfected TB- controls. The results reported here suggested that M. bovis manipulates host immune response by reducing the production of immune system proteins. However, as infection progresses, wild boar immune response recover to limit pathogen multiplication and promote survival that also facilitates pathogen transmission.

			Action	DRS
	BBFhaB.FASTA	Fasta
	BPFhaB.FASTA	Fasta
	DJ_FHA_BB_16O_AspN.baf	Other
	DJ_FHA_BB_16O_AspN.mgf	Mgf
	DJ_FHA_BB_16O_AspN.mzid.gz	Mzid

Dataset Information

The Bordetella pertussis and Bordetella bronchiseptica filamentous hemagglutinins are processed at different sites

Dataset's files

Publications

<i>Bordetella pertussis</i> and <i>Bordetella bronchiseptica</i> filamentous hemagglutinins are processed at different sites.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets