Proteomics

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N-glycoproteomic analyses from different insect species reveal conserved and unique glycoproteins


ABSTRACT: N-glycosylation is one of the most abundant and conserved protein modifications in eukaryotes. This modification serves various important functions, such as protein folding and cellular attachment, but also modulation of a protein’s function. Recently, it has been shown that N-glycosylation of proteins plays a vital role in insect development and survival, which makes it an interesting target for pest control. Despite the importance of protein N-glycosylation in insects, not much is known about insect N-glycoproteomes. Here, we report on the N-glycoproteomes of three major pest insects spanning different insect orders; Drosophila melanogaster (Diptera), Tribolium castaneum (Coleoptera) and Acyrthosiphon pisum (Hemiptera). The number of identified N-glycosylation sites ranged from 889 in T. castaneum, to 941 in D. melanogaster and 1,338 in A. pisum. Comparison between the different insect species revealed both conserved and species-specific glycoproteins. The functionality of the insect glycoproteins together with the conservation of the N-glycosites throughout evolution are discussed. This information can help in the elaboration of novel pest insect control strategies based on interference in insect glycosylation.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Buchnera Aphidicola Subsp. Acyrthosiphon Pisum (acyrthosiphon Pisum Symbiotic Bacterium) Tetropium Castaneum Drosophila Melanogaster (fruit Fly)

TISSUE(S): Whole Body

SUBMITTER: An Staes  

LAB HEAD: Guy Smagghe

PROVIDER: PXD008745 | Pride | 2018-06-18

REPOSITORIES: Pride

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Publications

Evolutionarily conserved and species-specific glycoproteins in the N-glycoproteomes of diverse insect species.

Scheys Freja F   Van Damme Els J M EJM   De Schutter Kristof K   Staes An A   Gevaert Kris K   Smagghe Guy G  

Insect biochemistry and molecular biology 20180612


N-glycosylation is one of the most abundant and conserved protein modifications in eukaryotes. The attachment of N-glycans to proteins can modulate their properties and influences numerous important biological processes, such as protein folding and cellular attachment. Recently, it has been shown that protein N-glycosylation plays a vital role in insect development and survival, which makes the glycans an interesting target for pest control. Despite the importance of protein N-glycosylation in i  ...[more]

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