Proteomics

Dataset Information

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Proteomic study of Anton Chekhov' letters


ABSTRACT: Five different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in A. Chekhov’s museum in Melikhovo (nearby Moscow), have been analyzed by applying to these surfaces EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C8 resins) diskettes. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectrometry. The environmental microbiota present on samples and the M. ycobacterium Tuberculosis tuberculosis strain were described by a meta-proteomics approach. Eight identified M. Tuberculosis tuberculosis proteins confirmed the presence of the bacterium and the cause of Chekhov death, in addition to several sequenced peptides belonging to other bacterial species. The human plasma proteins and human keratins, detected on a tiny blood spot on the shirt, demonstrated the power of the combined approach.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mycobacterium Tuberculosis K

SUBMITTER: Alfonsina D'Amato  

LAB HEAD: Alfonsina D'Amato

PROVIDER: PXD008786 | Pride | 2018-07-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
17_10_28_A1.mgf Mgf
17_10_28_A1.raw Raw
17_10_28_A2.mgf Mgf
17_10_28_A2.raw Raw
17_10_28_A3.mgf Mgf
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Five different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in the State Literary-Memorial Museum-Reserve A. P. Chekhov Melikhovo (nearby Moscow), have been analyzed by applying EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C<sub>8</sub> resins) diskettes to these surfaces. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectro  ...[more]

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