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Peptidomics of Human Hypothalamic neurons

ABSTRACT: The study of peptide secretion, and identifying its control requires the use of ELISAs for measuring their levels pre and post stimulation. In place of ELISA's an LC-MS methdology was developed first to identify the correct peptides generated by the neurons, which could then be used to measure the peptide products by targetted LC-MS analysis. The Extraction was also performed on human hypothalamic tissue obtained from a tissue bank. Samples were analysed by nano LC-MS, and searched using Peaks 8.5.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Paraventricular Nucleus Of Hypothalamus, Cerebral Cortex, Hypothalamic Nucleus, Primary Cerebral Cortical Neuronï¿½, Arcuate Nucleus

SUBMITTER: Richard Kay

LAB HEAD: Florian Merkle

PROVIDER: PXD008795 | Pride | 2018-08-22

REPOSITORIES: Pride

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			Action	DRS
	16070818.mgf	Mgf
	16070818.pride.mgf.gz	Mgf
	16070818.raw	Raw
	16070819.mgf	Mgf
	16070819.pride.mgf.gz	Mgf

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