Dataset Information

SILAC-based quantitative proteomic analysis of the livers of spontaneous obese and diabetic rhesus monkeys

ABSTRACT: Type 2 diabetes mellitus (TM) is a severely metabolic disorder that affects above 10% worldwide population. Obesity is a major cause of insulin resistance and contributes to the development of TM. Liver is an essential metabolic organ that plays crucial roles in the pathogenesis of obesity and diabetes. However, the underlying mechanisms of liver in the transition of obesity to diabetes are not fully understood. Nonhuman primate (NHP) rhesus monkey is an appropriate animal for research of human diseases. Here, we first screened and selected three individual spontaneous and diabetic rhesus monkeys. Interestingly, the diabetic monkeys were obese with high BMI at beginning, but gradually lost their body weight during one-year observation. Furthermore, we performed a SILAC-based quantitative proteomics to identify proteins and signaling pathways with altered expression in the liver of obese and diabetic monkeys. Totally, 3509 proteins were identified and quantified, and of which 185 proteins displayed altered expression level. GO analysis revealed that the expression of proteins involved in fatty acids β-oxidation and galactose metabolism was increased in obese monkeys; while proteins involved in oxidative phosphorylation (OXPHOS) and branched chain amino acid (BCAA) degradation was upregulated in diabetic monkeys. In addition, we observed a mild impaired mitophagy and apoptosis in the liver of diabetic monkeys, suggesting a dysfunction of mitochondria and liver injury in the late onset of diabetics. Taken together, our liver proteomics may reveal a distinct metabolic transition from fatty acids β-oxidation in obese monkey to BCAA degradation in diabetic monkeys.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Macaca Mulatta (rhesus Macaque)

TISSUE(S): Liver

SUBMITTER: Jing Gao

LAB HEAD: Zhou Hu

PROVIDER: PXD008825 | Pride | 2019-08-26

REPOSITORIES: pride

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			Action	DRS
	20150312_Zhangzg_Macaca_CK1_01.raw	Raw
	20150312_Zhangzg_Macaca_CK1_02.raw	Raw
	20150312_Zhangzg_Macaca_CK1_03.raw	Raw
	20150312_Zhangzg_Macaca_CK1_04.raw	Raw
	20150312_Zhangzg_Macaca_CK1_05.raw	Raw

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SILAC-based quantitative proteomic analysis of the livers of spontaneous obese and diabetic rhesus monkeys.

Wang Junlong J Xu Shimeng S Gao Jing J Zhang Linqiang L Zhang Zhiguo Z Yang Wenhui W Li Yunhai Y Liao Shasha S Zhou Hu H Liu Pingsheng P Liang Bin B

American journal of physiology. Endocrinology and metabolism 20180417 2

Type 2 diabetes mellitus (T2DM) is a severe metabolic disorder that affects more than 10% of the population worldwide. Obesity is a major cause of insulin resistance and contributes to the development of T2DM. Liver is an essential metabolic organ that plays crucial roles in the pathogenesis of obesity and diabetes. However, the underlying mechanisms of liver in the transition of obesity to diabetes are not fully understood. The nonhuman primate rhesus monkey is an appropriate animal for researc ...[more]

PMID: 29664677

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