Dataset Information

PSI supercomplex copurification using PSI His-tag

ABSTRACT: Chlamydomonas reinhardtii PSI-Cyt b6f complexes from sucrose density gradient fractions were identified by western blot and SMF spectroscopy, but showed high heterogeneity, as evident from the multiple bands present in the Coomassie stained SDS gel and original EM micrographs. Therefore potential PSI-Cyt b6f supercomplexes were enriched by a subsequent PsaA HisTag purification step. Only this additional enrichment enabled the identification of novel PSI supercomplexes, although the sample remained far from being homogeneous. This dataset describes the quantitative mass spectrometric analysis of the enriched PSI supercomplex fraction in comparison to unpurified samples using metabolic 15N labeling.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Photosynthetic Cell

SUBMITTER: Stefan Schulze

LAB HEAD: Michael Hippler

PROVIDER: PXD008857 | Pride | 2018-10-03

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	14N_DSS-__unified_percolator_validated_accepted.u.json.u.json	Other
	15N_DSS-__unified_percolator_validated_accepted.u.json.u.json	Other
	15N_DSS-__unified_percolator_validated_accepted.u_merged.csv	Csv
	1_N_DSS-__unified_percolator_validated_accepted.u_merged.u_merged.csv	Csv
	JST_10092016_PSA-HisTag_SCneN14_SCpurE3N15_01.mzML	Mzml

Items per page:

1 - 5 of 9

Publications

Structure of a PSI-LHCI-cyt b<sub>6</sub>f supercomplex in <i>Chlamydomonas reinhardtii</i> promoting cyclic electron flow under anaerobic conditions.

Steinbeck Janina J Ross Ian L IL Rothnagel Rosalba R Gäbelein Philipp P Schulze Stefan S Giles Nichole N Ali Rubbiya R Drysdale Rohan R Sierecki Emma E Gambin Yann Y Stahlberg Henning H Takahashi Yuichiro Y Hippler Michael M Hankamer Ben B

Proceedings of the National Academy of Sciences of the United States of America 20180925 41

Photosynthetic linear electron flow (LEF) produces ATP and NADPH, while cyclic electron flow (CEF) exclusively drives photophosphorylation to supply extra ATP. The fine-tuning of linear and cyclic electron transport levels allows photosynthetic organisms to balance light energy absorption with cellular energy requirements under constantly changing light conditions. As LEF and CEF share many electron transfer components, a key question is how the same individual structural units contribute to the ...[more]

PMID: 30254175

Dataset Information

PSI supercomplex copurification using PSI His-tag

Dataset's files

Publications

Structure of a PSI-LHCI-cyt b<sub>6</sub>f supercomplex in <i>Chlamydomonas reinhardtii</i> promoting cyclic electron flow under anaerobic conditions.

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