Proteomics

Dataset Information

0

Intensity-based absolute quantification (iBAQ) of components of photosystem I monomers and dimers.


ABSTRACT: Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Cell Suspension Culture, Photosynthetic Cell

SUBMITTER: Martin Scholz  

LAB HEAD: Michael Hippler

PROVIDER: PXD026990 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AVM_20210610_ML2_5B.raw Raw
Creinhardtii_281_v5.6_Mito_Chloro_29052020.fasta Fasta
LM_PSI-dimer_13042021.raw Raw
LM_PSI-mono_13042021.raw Raw
Samples.txt Txt
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Publications


Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer, comprising 40 protein subunits with 118 transmembrane helices that provide scaffold for 568 pigments. Cryogenic electron microsc  ...[more]

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