Dataset Information

LC MS/MS comparative proteomic analysis of Porphyromonas gingivalis treated with ranitidine bismuth citrate

ABSTRACT: Label-free quantification of quantitative proteomics was performed to to extensively understand the effects of bismuth drugs (25 µM RBC) on Porphyromonas gingivalis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Porphyromonas Gingivalis Atcc 33277

SUBMITTER: Tianfan Cheng

LAB HEAD: Lijian Jin

PROVIDER: PXD008869 | Pride | 2024-10-23

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 115

Publications

Bismuth drugs tackle Porphyromonas gingivalis and attune cytokine response in human cells.

Cheng Tianfan T Lai Yau-Tsz YT Wang Chuan C Wang Yi Y Jiang Nan N Li Hongyan H Sun Hongzhe H Jin Lijian L

Metallomics : integrated biometal science 20190701 7

Periodontitis is the leading cause of severe tooth loss and edentulism in adults worldwide and is closely linked to systemic conditions such as diabetes and cardiovascular disease. Porphyromonas gingivalis is the key pathogen in periodontitis. Herein, we provided the first evidence that bismuth drugs suppress P. gingivalis in its planktonic, biofilm, and intracellular states. In total, 42 bismuth-associated proteins were identified including its major virulent factors (e.g., gingipains, hemagglu ...[more]

PMID: 31179464

Similar Datasets

Project description:Porphyromonas gingivalis is a major pathogen associated with the microbial biofilm-mediated disease chronic periodontitis. P. gingivalis has an obligate requirement for iron and protoporphyrin IX which it satisfies by transporting heme and iron liberated from the human host. The level of cellular iron in P. gingivalis affects the expression of a distinct iron-associated regulon of 64 genes and low iron invokes an iron sparing response. Iron homeostasis is usually mediated in Gram-negative bacteria at the transcriptional level by the Ferric Uptake Regulator (Fur). There is a single predicted P. gingivalis Fur superfamily orthologue named Har (heme associated regulator) that lacks the conserved metal binding residues found in other Fur orthologues. We show that Har binds both heme and ferrous iron resulting in a conformational change in the protein. Har was unable to complement the Escherichia coli H1780 fur mutant and there was no change in cellular metal content in a P. gingivalis Har mutant compared with the wild-type. The Har regulon of 44 genes is not predicted to play a role in iron homeostasis. Together these data indicated that Har does not regulate iron homeostasis in P. gingivalis. However, Har was required for heme-responsive biofilm development and its regulon overlapped P. gingivalis regulons previously identified after growth in heme limitation or as a homotypic biofilm. P. gingivalis is unique as an iron-dependent Gram-negative bacterium with a single heme-binding Fur superfamily orthologue, Har, that does not regulate iron homeostasis. Paired samples were compared on the same microarray using a two-colour system. A total of 6 paired microarray hybridizations were performed representing 6 biological replicates, where a balanced dye design was used, with the overall analysis including three microarrays where P. gingivalis 33277 samples were labeled with Cy3 and the paired ECR455 samples were labeled with Cy5 and three other microarrays where samples were labeled with the opposite combination of fluorophores.

			Action	DRS
	Jin_5Jul2017_PgRBC_1.raw	Raw
	Jin_5Jul2017_PgRBC_2.raw	Raw
	Jin_5Jul2017_PgRBC_3.raw	Raw
	Jin_5Jul2017_Pg_1.raw	Raw
	Jin_5Jul2017_Pg_2.raw	Raw