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Mango-EcoliBDP - Mango: A General Tool for Collision Induced Dissociation-Cleavable Cross-Linked Peptide Identification

ABSTRACT: Dataset presented in Mango manuscript. Contains Ecoli cross-linked with BDP-NHP. Demonstration of whole proteome in-vivo cross-linking without an instrument capable of serial fragmentation.

INSTRUMENT(S): LTQ FT, Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Jared Mohr

LAB HEAD: James Bruce

PROVIDER: PXD008975 | Pride | 2019-02-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	012618_Eco1_noXL_mango_1.raw	Raw
	012618_Eco_noXL_mangoSummary.xls	Xls
	051917_Eco1_F10_NCE30_4hr_1.ms2	Other
	051917_Eco1_F10_NCE30_4hr_1.mzXML	Mzxml
	051917_Eco1_F10_NCE30_4hr_1.pep.xml	Pepxml

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Publications

Mango: A General Tool for Collision Induced Dissociation-Cleavable Cross-Linked Peptide Identification.

Mohr Jared P JP Perumalla Poorna P Chavez Juan D JD Eng Jimmy K JK Bruce James E JE

Analytical chemistry 20180427 10

Chemical cross-linking combined with mass spectrometry provides a method to study protein structures and interactions. The introduction of cleavable bonds in a cross-linker provides an avenue to decouple released peptide masses from their precursor species, greatly simplifying the downstream search, allowing for whole proteome investigations to be performed. Typically, these experiments have been challenging to carry out, often utilizing nonstandard methods to fully identify cross-linked peptide ...[more]

PMID: 29676898

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