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HeLa Cells Cross-Linked In Vivo versus as a Lysate

ABSTRACT: HeLa Cells were cross-linked with PIR either in vivo or as a lysate.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Cell Culture

SUBMITTER: Andrew Keller

LAB HEAD: James E. Bruce

PROVIDER: PXD013063 | Pride | 2019-07-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	112017_LysXL_control_F10_KCF.pep.xml	Pepxml
	112017_LysXL_control_F10_KCF.raw	Raw
	112017_LysXL_control_F11_14_KCF.pep.xml	Pepxml
	112017_LysXL_control_F11_14_KCF.raw	Raw
	112017_LysXL_control_F6_7_KCF.pep.xml	Pepxml

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Publications

Prediction of an Upper Limit for the Fraction of Interprotein Cross-Links in Large-Scale In Vivo Cross-Linking Studies.

Keller Andrew A Chavez Juan D JD Felt Kevin C KC Bruce James E JE

Journal of proteome research 20190717 8

Chemical cross-linking and mass spectrometry is of growing use for establishment of distance constraints on protein conformations and interactions. Whereas intraprotein cross-links can arise from proteins in isolation, interprotein cross-links reflect proximity of two interacting proteins in the sample. Prediction of expected ratios of the number of interprotein to intraprotein cross-links is hindered by lacking comprehensive knowledge on the interactome network and global occupancy levels for a ...[more]

PMID: 31267744

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