Dataset Information

Proteomic analysis of California sea lion urine

ABSTRACT: It is often necessary to demonstrate measurement capabilities in fields not already using state of the art bioanalytical platforms in order to encourage metrological modernization and adoption of such technologies. Marine mammal proteomics is a burgeoning field with diverse stakeholders and rapid investment in *omic platforms (e.g., genome sequencing). Previous marine mammal proteomic studies have focused on tissues and biofluids such as plasma, serum and cerebrospinal fluid. Despite its utility in human research, to date there has not been a comprehensive proteomic analysis of marine mammal urine. Using best practice sample collection, a retrospective urine sample set was generated. These results serve as a proof of principle for development of assays in other species.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Zalophus Californianus (california Sealion)

TISSUE(S): Urine

SUBMITTER: Benjamin Neely

LAB HEAD: Benjamin Adam Neely

PROVIDER: PXD009019 | Pride | 2018-06-06

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2017-5-25_CSL_03_redux - F001946.pride.mztab.gz	Mztab
	2017-5-25_CSL_03_redux-F001946.dat	Other
	2017-5-25_CSL_03_redux-F001946.mzid.gz	Mzid
	2017-5-25_CSL_03_redux.mgf	Mgf
	2017-5-25_CSL_03_redux.pride.mgf.gz	Mgf

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Publications

Proteomic Analysis of Urine from California Sea Lions ( Zalophus californianus): A Resource for Urinary Biomarker Discovery.

Neely Benjamin A BA Prager Katherine C KC Bland Alison M AM Fontaine Christine C Gulland Frances M FM Janech Michael G MG

Journal of proteome research 20180828 9

Urinary markers for the assessment of kidney diseases in wild animals are limited, in part, due to the lack of urinary proteome data, especially for marine mammals. One of the most prevalent kidney diseases in marine mammals is caused by Leptospira interrogans, which is the second most common etiology linked to stranding of California sea lions ( Zalophus californianus). Urine proteins from 11 sea lions with leptospirosis kidney disease and eight sea lions without leptospirosis or kidney disease ...[more]

PMID: 30113852

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Project description:Urinary proteins provide valuable insights into renal health, with implications spanning human and domestic animal veterinary medical research. However, the field of marine mammal medicine lacks comprehensive studies on urine protein composition. This research aimed to fill this gap by 1) selecting an optimal search strategy that yields the highest number of protein families in the bottlenose dolphin urine based on post-translational modifications (PTMs), 2) describing the urine proteome of wild bottlenose dolphins (Tursiops truncatus) in the Gulf of the Mexico, USA, considering sex (females vs. males), site (Barataria Bay, LA vs. Sarasota Bay, FL), and comparing with California sea lions (Zalophus californianus). Ten urine samples (Barataria Bay, LA: N= 6; Sarasota Bay, FL: N=4) collected during 2023 catch-and-release heath assessment were proteolytically digested and analyzed using an UltiMate 3000 Nano LC and Fusion Lumos Orbitrap mass spectrometer. A 2-step search strategy, incorporating dehydroalanine formation and semi-trypsin on the list of unassigned spectra, significantly increased the number of identified protein families by an average of 6.2% compared to the 1-step strategy (P < 0.001, t = -8.32). The top 30 proteins in bottlenose dolphin urine were ranked according to an exponentially modified protein abundance index for comparison based on sex, site, and species. There were no significant differences in urine proteins between sexes or sites (padj > 0.05), although there was sperm contribution in two of the male bottlenose dolphin urine samples. Two putative antimicrobial proteins (cathelicidin and lysozyme) were identified and found to be abundant in bottlenose dolphin urine, similar to California sea lions. The study also identified 27 potential markers of acute kidney injury and 12 regulators of kidney stone formation. This study established a reference database of urinary proteins from bottlenose dolphins, aiding future research in monitoring and evaluating renal health in marine mammals.