Dataset Information

NanoLC-MSMS TMT comparison of circulated and unstimulated in vitro cultured reticulocytes

ABSTRACT: Triplicate samples of unstimulated in vitro culture-derived reticulocytes (derived from CD34+ cells) and cultured reticulocytes circulated overnight in an ex vivo circulation system were analysed through TMT-based quantitative proteomics to explore the impact of shear stress on reticulocyte protein abundance.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Culture, Reticulocyte

SUBMITTER: Pedro Moura

LAB HEAD: Ashley Mark Toye

PROVIDER: PXD009024 | Pride | 2018-08-03

REPOSITORIES: Pride

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Publications

Non-muscle myosin II drives vesicle loss during human reticulocyte maturation.

Moura Pedro L PL Hawley Bethan R BR Mankelow Tosti J TJ Griffiths Rebecca E RE Dobbe Johannes G G JGG Streekstra Geert J GJ Anstee David J DJ Satchwell Timothy J TJ Toye Ashley M AM

Haematologica 20180803 12

The process of maturation of reticulocytes into fully mature erythrocytes that occurs in the circulation is known to be characterized by a complex interplay between loss of cell surface area and volume, removal of remnant cell organelles and redundant proteins, and highly selective membrane and cytoskeletal remodeling. However, the mechanisms that underlie and drive these maturational processes <i>in vivo</i> are currently poorly understood and, at present, reticulocytes derived through <i>in vi ...[more]

PMID: 30076174

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			Action	DRS
	0609176PlexTMT.pep.xml	Pepxml
	0609176PlexTMT.xlsx	Xlsx
	0609176PlexTMT_PS.msf	Msf
	Fraction_10.raw	Raw
	Fraction_11.raw	Raw