Dataset Information

Human T-cells EVs LC-MSMS - Extracellular Vesicle Subtypes Released From Activated or Apoptotic T-Lymphocytes Carry a Specific and Stimulus-Dependent Protein Cargo

ABSTRACT: As it is likely that different stimuli promote the release of distinct EV populations, we analyzed EVs from human lymphocytes considering the respective release stimuli (activation vs. apoptosis induction). Morphology and size were analyzed by electron microscopy and nanoparticle tracking analysis. The protein content of these vesicles was analyzed by bidimensional gel electrophoresis followed by mass spec and western blot.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, T Cell

SUBMITTER: Christine Tucher

LAB HEAD: Dr. Martin Schiller

PROVIDER: PXD009074 | Pride | 2018-03-15

REPOSITORIES: Pride

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			Action	DRS
	X-15-221-A06.mzid.gz	Mzid
	X-15-221-A06.mzid_X-15-221-A06.MGF	Mzid
	X-15-221-A06.mzid_X-15-221-A06.pride.mgf.gz	Mzid
	X-15-221-A06.pride.mztab.gz	Mztab
	X-15-221-A06.raw	Raw

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Extracellular Vesicle Subtypes Released From Activated or Apoptotic T-Lymphocytes Carry a Specific and Stimulus-Dependent Protein Cargo.

Tucher Christine C Bode Konrad K Schiller Petra P Claßen Laura L Birr Carolin C Souto-Carneiro Maria Margarida MM Blank Norbert N Lorenz Hanns-Martin HM Schiller Martin M

Frontiers in immunology 20180315

Extracellular vesicles (EVs) are released from nearly all mammalian cells and different EV populations have been described. Microvesicles represent large EVs (LEVs) released from the cellular surface, while exosomes are small EVs (SEVs) released from an intracellular compartment. As it is likely that different stimuli promote the release of distinct EV populations, we analyzed EVs from human lymphocytes considering the respective release stimuli (activation Vs. apoptosis induction). We could cle ...[more]

PMID: 29599781

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