Dataset Information

Transcriptome and excretory–secretory proteome of infective-stage larvae of Gnathostoma spinigerum reveal potential immunodiagnostic targets for development Parasite

ABSTRACT: This paper *potentially* provides a useful transcriptomic and proteomic resource for the parasitic nematode Gnathostoma spinigerum. Most importantly, the paper gives not only proteomics data but also immunoblot data for antigenic responses of infected humans against G. spinigerum proteins that were probably secreted into human hosts in vivo. These data are potentially useful to biomedicine.

INSTRUMENT(S): Bruker Daltonics micrOTOF series

ORGANISM(S): Gnathostoma Spinigerum

TISSUE(S): Whole Body

SUBMITTER: onrapak reamtong

LAB HEAD: Poom Adisakwattana

PROVIDER: PXD013551 | Pride | 2019-07-22

REPOSITORIES: Pride

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Transcriptome and excretory-secretory proteome of infective-stage larvae of the nematode Gnathostoma spinigerum reveal potential immunodiagnostic targets for development.

Nuamtanong Supaporn S Reamtong Onrapak O Phuphisut Orawan O Chotsiri Palang P Malaithong Preeyarat P Dekumyoy Paron P Adisakwattana Poom P

Parasite (Paris, France) 20190605

<h4>Background</h4>Gnathostoma spinigerum is a harmful parasitic nematode that causes severe morbidity and mortality in humans and animals. Effective drugs and vaccines and reliable diagnostic methods are needed to prevent and control the associated diseases; however, the lack of genome, transcriptome, and proteome databases remains a major limitation. In this study, transcriptomic and secretomic analyses of advanced third-stage larvae of G. spinigerum (aL3Gs) were performed using next-generatio ...[more]

PMID: 31166909

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Project description:The experiment was carried out during two vegetation seasons from 25th of April to 10th of September. Two Polish potato cultivars, the drought-tolerant Gwiazda and drought-sensitive Oberon were used. Selected tubers with transverse diameters of 3 - 4 cm were pre-sprouted for 2 weeks before planting. Plants were grown in a vegetation hall in pots filled with a thin layer of gravel on the bottom and the universal vegetable soil substrate ‘Hollas’ (Agaris Polska Ltd., Poland) produced from peat with the addition of chalk at a pH range of 5.5-6.5. Additionally, in phase 20 of the BBCH-scale of plant development, MIS-3 (Intermag) fertilizer was applied. Water content (WC) in volumetric basis in soil pots was measured according Black (1965). Weather conditions during the years of study were monitored using a Weather Campbell Station (Campbell Scientific Inc.) located in close proximity, and a thermohygrograph placed between pots. Meteorological data of air temperature, the photosynthetically active radiation, and humidity were comparable in the years of study and favorable for potato development. Three weeks after the initiation of the tuberisation phase (56 DAP), plants were divided into 3 groups, each consisting of 6 plants. The first group of plants was subjected to soil drought (remained without irrigation, day/night temperature 22°C/18°C), the second one to high temperature (day/night temperature 38°C/25°C) and the third one was watered according to needs and at optimal temperature (control plants, day/night temperature 22°C/18°C). Stress application lasted 14 days and finished at 70 DAP. During this period, plants were placed in phytotron equipped with six Hortilux Schreder Lamps with Philips light bulbs of 1600 W each. Air humidity was in the range 65-70%. WC under 14 days of soil drought reached 30% (v/v), and remained 80% (v/v) in control and high temperature conditions. During the recovery period, after 14 days of stress treatment, WC reached control levels. Plant root material for proteomic research was collected on the 14th day of stress treatment (70 DAP).

			Action	DRS
	GS10_BB2_01_2293.baf_idx	Other
	GS10_BB2_01_2293.dat	Other
	GS10_BB2_01_2293.mgf	Mgf
	GS11_BB3_01_2294.baf_idx	Other
	GS11_BB3_01_2294.dat	Other