Dataset Information

PROPEP3-VENUS - The plant cell wall integrity maintenance and immune signaling systems cooperate to control stress responses in Arabidopsis thaliana

ABSTRACT: The structure and composition of plant cell walls are modified to accommodate the needs of the cell and in response to environmental stimuli. Growth, development, and defense may demand potentially conflicting functional cell wall requirements, and thus modifications of the cell wall are tightly controlled in an adaptive manner. These modifications are mediated by a dedicated cell wall integrity (CWI) maintenance mechanism. We investigated the responses to cell wall damage (CWD) that compromise CWI and the underlying mechanisms in Arabidopsis thaliana. Inhibitor- and enzyme-triggered CWD induced similar, turgor-sensitive stress responses. Genetic analysis showed that the receptor-like kinase (RLK) FEI2 and the plasma membrane-localized mechano-sensitive Ca2+- channel MCA1 function downstream of the RLK THE1 in CWD perception. Phenotypic clustering with 27 genotypes identified a core group of RLKs and ion channels required for activation of CWD responses. In contrast, the responses were repressed by pattern-triggered immunity (PTI) signaling components including the receptors for plant elicitor peptides (AtPeps) PEPR1 and PEPR2 (PEPR1/2). Application of AtPep1 and AtPep3 repressed CWD-induced phytohormone accumulation in a concentration dependent manner. CWD induced the expression of both PROPEP1 and PROPEP3 as well as the release of a PROPEP3 fusion protein into the growth medium. These results suggest that AtPep-mediated signaling suppresses CWD-induced defense responses. If key PTI signaling elements acting downstream of PEPR1/2 are dysfunctional, suppression of CWD-induced responses is alleviated, thus compensating for the impairment.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seed

SUBMITTER: Davi Fonseca

LAB HEAD: Thorsten Hamann

PROVIDER: PXD009153 | Pride | 2018-07-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ArabidopsisThaliana_2017-09-03_uniprot_sp-and-tr_isoforms_UP000006548.fasta	Fasta
	Contaminants_ProperHeaders_InclusingAspNs-WithoutGFP.fasta	Fasta
	DMSO25kDaband.msf	Msf
	DMSO25kDaband.msfView	Msf
	DMSO25kDaband.pdResult	Other

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The plant cell wall integrity maintenance and immune signaling systems cooperate to control stress responses in <i>Arabidopsis thaliana</i>.

Engelsdorf Timo T Gigli-Bisceglia Nora N Veerabagu Manikandan M McKenna Joseph F JF Vaahtera Lauri L Augstein Frauke F Van der Does Dieuwertje D Zipfel Cyril C Hamann Thorsten T

Science signaling 20180626 536

Cell walls surround all plant cells, and their composition and structure are modified in a tightly controlled, adaptive manner to meet sometimes opposing functional requirements during growth and development. The plant cell wall integrity (CWI) maintenance mechanism controls these functional modifications, as well as responses to cell wall damage (CWD). We investigated how the CWI system mediates responses to CWD in <i>Arabidopsis thaliana</i> CWD induced by cell wall-degrading enzymes or an inh ...[more]

PMID: 29945884

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