Dataset Information

In vitro blood-brain barrier model proteome influenced by silver nanoparticles

ABSTRACT: Silver nanoparticles (AgNP) have been reported to penetrate the central nervous system and induce neurotoxicity. However, there is paucity in understanding the toxicity of AgNPs and their effect on the blood-brain barrier (BBB) including the underlying molecular mechanism(s) of action. Using an in vitro BBB model and mass spectrometry based proteomics we investigated the alteration in the proteome pathways of the brain endothelial cells and astrocytes exposed to AgNPs for 24 and 48 hours.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain Microvascular Endothelial Cell, Cell Culture, Astrocyte

DISEASE(S): Disease Free

SUBMITTER: Vladimir Gorshkov

LAB HEAD: Frank Kjeldsen

PROVIDER: PXD009197 | Pride | 2018-11-07

REPOSITORIES: Pride

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Silver nanoparticle-induced expression of proteins related to oxidative stress and neurodegeneration in an in vitro human blood-brain barrier model.

Khan Asif Manzoor AM Korzeniowska Barbara B Gorshkov Vladimir V Tahir Muhammad M Schrøder Henrik H Skytte Lilian L Rasmussen Kaare Lund KL Khandige Surabhi S Møller-Jensen Jakob J Kjeldsen Frank F

Nanotoxicology 20190109 2

Silver nanoparticles (AgNPs) have been reported to penetrate the central nervous system (CNS) and induce neurotoxicity. However, there is a paucity of understanding of the toxicity of AgNPs and their effect on the blood-brain barrier (BBB) including the underlying molecular mechanism(s) of action. Such information is important for the formulation of new strategies for delivery of biological therapeutics to central nervous system (CNS) targets. Using an in vitro BBB model and mass spectrometry-ba ...[more]

PMID: 30623748

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	Astro48.pdResult	Other
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