Dataset Information

Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes

ABSTRACT: Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This projected investigated molecular mechanisms of TEGDMA toxicity by identifying time- and dose dependent effects on the proteome of human THP-1 monocytes. Effects of different concentrations (0.07mM-5mM) and exposure times (0-72h) of TEGDMA on cell viability, proliferation, and morphology were determined by a real-time viability assay, automated cell counting, and electron microscopy, and laid the fundament for choice of exposure scenarios in the proteomic experiments. Cells were metabolically labelled (SILAC), and exposed to 0.3mM or 2.5mM TEGDMA for 6h or 16h prior to LC-MS/MS analyses. Regulated proteins were analysed with the STRING database. Cells exposed to 0.3mM TEGDMA showed increased viability, and time-dependent upregulation of proteins associated with stress/oxidative stress, autophagy, and cytoprotective functions. Cells exposed to 2.5mM TEGDMA showed diminished viability, and a protein expression profile associated with oxidative stress, DNA-damage, mitochondrial dysfunction and cell-cycle inhibition. Altered expression of immune genes was observed in both groups.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

SUBMITTER: Jack-Ansgar Bruun

LAB HEAD: Ulf Örtengren

PROVIDER: PXD009206 | Pride | 2018-07-31

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	160603_BWNilsen_SILAC_E1.raw	Raw
	160603_BWNilsen_SILAC_E2.raw	Raw
	160603_BWNilsen_SILAC_E3.raw	Raw
	160603_BWNilsen_SILAC_E4.raw	Raw
	160603_BWNilsen_SILAC_F1.raw	Raw

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Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes.

Nilsen Bo W BW Simon-Santamaria Jaione J Örtengren Ulf U Jensen Einar E Bruun Jack-Ansgar JA Michelsen Vibeke B VB Sørensen Karen K KK

European journal of oral sciences 20180727 5

Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resin-based dental materials. This study investigated the molecular mechanisms of TEGDMA toxicity by identifying its time- and dose-dependent effects on the proteome of human THP-1 monocytes. The effects of different concentrations (0.07-5 mM) and exposure times (0-72 h) of TEGDMA on cell viability, proliferation, and morphology were determined using a real-time viability assay, automated cell counting, and electron microscop ...[more]

PMID: 30051916

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