Dataset Information

Human protein phosphorylation analysis

ABSTRACT: Mycobacterium tuberculosis (Mtb) protein kinase G (PknG) is a critical protein which can inhibit the maturation of phagosome and its fusion with the lysosome, providing Mtb’s latency and survival in the host. However, its signaling pathways and functions in the human body are still unclear and needed to be further studied. In our research, we used the human protein microarray to study the proteins interacting with PknG and have found a list of proteins that are potentially related to PknG in the host, which may contribute to the infection of Mtb. Among these positive hits, we found human protein CypA has strong interaction with PknG. We further validated the interaction between PknG and CypA in vitro and in vivo and investigated its role in the infection of macrophage cells. Finally, we found that PknG can significantly down-regulate protein expression level of CypA through phosphorylation in site of T107 as well as its induction of inflammatory response through NF-κB and ERK1/2 pathways. Meanwhile, the survival of Mycobacterium smegmetis (Msm) in the macrophages with overexpressing PknG is also improved with lessen of cytokines expression. Our results provided a resourceful research base of PknG in regards to various target human proteins and offered an insightful understanding of PknG’s function in the host.

INSTRUMENT(S): ultraflex

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Fanlin Wu

LAB HEAD: Sheng-Ce Tao

PROVIDER: PXD009237 | Pride | 2018-10-10

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20180313_BA5_01_6125.mgf	Mgf
	20180313_BA5_01_6125.pride.mgf.gz	Mgf
	20180313_BA5_01_6125.u2	Other
	20180313_BA5_01_6125.xml	Xml
	20180313_BA5_01_6125_1.u2	Other

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Publications

Global Profiling of PknG Interactions Using a Human Proteome Microarray Reveals Novel Connections with CypA.

Wu Fan-Lin FL Liu Yin Y Zhang Hai-Nan HN Jiang He-Wei HW Cheng Li L Guo Shu-Juan SJ Deng Jiao-Yu JY Bi Li-Jun LJ Zhang Xian-En XE Gao Hua-Fang HF Tao Sheng-Ce SC

Proteomics 20181024 23

Mycobacterium tuberculosis (Mtb) serine/threonine kinase PknG plays an important role in the Mtb-host interaction by facilitating the survival of Mtb in macrophages. However, the human proteins with which the PknG interacts, and the underlying molecular mechanisms are still largely unknown. In this study, a HuProt array is been applied to globally identify the host proteins to which PknG binds. In this way, 125 interactors are discovered, including a cyclophilin protein, CypA. This interaction b ...[more]

PMID: 30281201

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