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Mycobacterium tuberculosis whiB4 mutant expression

ABSTRACT: Comparison of gene expression profile of the whiB4 mutant strain of Mycobacterium tuberculosis with the wild type Mycobacterium tuberculosis H37RV Mtb WhiB4 mutant mRNA was compared with the mRNA of wtMtb H37RV under aerobic conditons Aerbic conditions OD600 nm of 0.4, MtbWhiB4KO vs wtMtb, biological replicates: 3 wt Mtb H37RV and 3 MtbWhiB4 KO

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Amit Singh

PROVIDER: E-GEOD-37840 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mycobacterium tuberculosis WhiB4 regulates oxidative stress response to modulate survival and dissemination in vivo.

Chawla Manbeena M Parikh Pankti P Saxena Alka A Munshi Mohamedhusen M Mehta Mansi M Mai Deborah D Srivastava Anup K AK Narasimhulu K V KV Redding Kevin E KE Vashi Nimi N Kumar Dhiraj D Steyn Adrie J C AJ Singh Amit A

Molecular microbiology 20120726 6

Host-generated oxidative stress is considered one of the main mechanisms constraining Mycobacterium tuberculosis (Mtb) growth. The redox-sensing mechanisms in Mtb are not completely understood. Here we show that WhiB4 responds to oxygen (O₂) and nitric oxide (NO) via its 4Fe-4S cluster and controls the oxidative stress response in Mtb. The WhiB4 mutant (MtbΔwhiB4) displayed an altered redox balance and a reduced membrane potential. Microarray analysis demonstrated that MtbΔwhiB4 overexpresses th ...[more]

PMID: 22780904

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