Proteomics

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Development of a parallel reaction monitoring-MS method to quantify IGF proteins in dogs: application to a canine case study of NICTH syndrome

ABSTRACT: Non–islet-cell tumor hypoglycemia (NICTH) is a rare paraneoplastic phenomenon seen in both dogs and humans. NICTH syndrome is derived from incompletely processed forms of insulin-like growth factor–II (IGF–II) by tumors, commonly named as big IGF–II. In the present study, a previously developed targeted PRM MS-based method for cats have been optimized and applied to simultaneously quantify the levels of IGF–I, IGF–II, and IGFBP–3, and for the first time, the levels of big IGF–II in dogs. This method allows the absolute quantification of these proteins using a mixture of QPrEST™ proteins previously designed for humans, reducing the variations due to the methodology, and the amount of serum needed for the analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Blood Cell, Blood Plasma

SUBMITTER: Alberto Valdés  

LAB HEAD: Emma M. Strage

PROVIDER: PXD009277 | Pride | 2018-11-04

REPOSITORIES: Pride

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Development of a Parallel Reaction Monitoring-MS Method To Quantify IGF Proteins in Dogs and a Case of Nonislet Cell Tumor Hypoglycemia.

Valdés Alberto A   Lewitt Moira M   Wiss Erica E   Ramström Margareta M   Strage Emma M EM  

Journal of proteome research 20181030 1

Nonislet-cell tumor hypoglycemia (NICTH) is a rare paraneoplastic phenomenon well described in dogs and humans. Tumors associated with NICTH secrete incompletely processed forms of insulin-like growth factor-II (IGF-II), commonly named big IGF-II. These forms have increased bioavailability and interact with the insulin and IGF-I receptor causing hypoglycemia and growth-promoting effects. Immunoassays designed for human samples have been used to measure canine IGF-I and -II, but they possess some  ...[more]

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