Dataset Information

A targeted proteomics strategy to identify markers of resistance in pea

ABSTRACT: Ascochyta blight, caused by Peyronellaea pinodes, is one of major diseases of pea worldwide. Only low levels of resistance are available in pea cultivars. Quantitative Trait Loci associated with resistance to this disease have been identified, but the resistant genes underlying these QTLs are unknown making difficult marker assisted selection. In complex traits involving protein modifications and changes in protein abundance, quantitative protein estimation can be useful as alternative markers for breeding. In this study, we have developed a targeted proteomic strategy to identify protein markers associated with resistance and explore the feasibility of using them in breeding selection. For such purpose, in a first step a list of target peptides was generated based on proteins highly related to P. pinodes resistance by using two genotypes showing extreme behavior. Subsequently, a second experiment was carried out in a population segregating for resistance. Those peptides constitutively more abundant in the resistant lines were selected as potential markers of resistance to P. pinodes. Based on the results obtained, attending to the functions and correlation among the significantly increased proteins in response to Ascochyta blight, we propose a mechanism of defense in pea. As far as we know, this is the first time that such targeted proteomic strategy (shotgun combined data-independent analysis) is used to identify markers of resistance to this disease.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Pisum Sativum (garden Pea)

TISSUE(S): Leaf

SUBMITTER: M.ANGELES CASTILLEJO

LAB HEAD: Maria Angeles Castillejo Sanchez

PROVIDER: PXD009351 | Pride | 2022-02-22

REPOSITORIES: Pride

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Publications

Quantitative Analysis of Target Peptides Related to Resistance Against Ascochyta Blight (Peyronellaea pinodes) in Pea.

Castillejo María-Ángeles MÁ Fondevilla-Aparicio Sara S Fuentes-Almagro Carlos C Rubiales Diego D

Journal of proteome research 20200210 3

Peyronellaea pinodes causes Ascochyta blight, one of the major diseases in pea worldwide. Cultivated pea plants have a low resistance to this disease. Although quantitative trait loci (QTLs) involved in the resistance to Ascochyta blight have been identified, the specific genes associated with these QTLs remain unknown, which makes marker-assisted selection difficult. Complex traits alter proteins and their abundance. Quantitative estimation of proteins in pea might therefor ...[more]

PMID: 32040328

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Project description:Ascochyta blight (AB) is a devastating and aggressive disease of field peas caused by multiple pathogens and the occurrence of the combinations of these pathogens forms a disease complex of which Peyronellaea pinodes, is the most aggressive. AB symptoms include necrotic lesions on all above ground parts of the plant, which can reduce crop yield by up to 70% and result in major economic losses globally. Crop resistance to the disease is reported to be quantitative and, although several genes have been reported to be AB response genes in Spanish pea genotypes, the lines or genotypes of the Australian field pea breeding program have not been investigated. Plants have specialized defence mechanisms that enable them respond to pathogen invasion, and this involves a cascade of genes which are differentially regulated upon infection. In this study, the time-course gene expression profiles of four Australian field pea genotypes including commercial varieties, known to have varying levels of AB resistance were investigated in response to P. pinodes infection. Infected and control plants at 0, 6, 24, 48, 72 and 96HPI were sampled for RNA seq. Mapping of transcriptome sequencing data against the field pea genome resulted in about the identification of c. 6,700 differentially expressed genes. Of the 21 AB resistance genes reported in previously published studies, only seven were present on the list of DEGs generated in this study. No significant pattern was identified in gene expression for the seven genes to differentiate resistant from susceptible genotypes. However, four key genes i.e., disease response protein, malate dehydrogenase, isoflavone 2 hydrogenase and Isoflavone reductase were shown to be associated with suppression of AB disease phenotype after 96HPI. Furthermore, GO functional annotation revealed several genes associated with defence including genes associated with the Salicylic acid pathway, reactive oxygen species, programmed cell death and hypersensitive response. Although the overall result showed a lack of strong resistance in the Australian pea genotypes as well as the fact that disease suppression does not directly equal resistance, we however were able to identify potential candidates that could be pyramided from exotic material into the breeding program to assess for improved resistance.

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Dataset Information

A targeted proteomics strategy to identify markers of resistance in pea

Dataset's files

Publications

Quantitative Analysis of Target Peptides Related to Resistance Against <i>Ascochyta</i> Blight (<i>Peyronellaea pinodes</i>) in Pea.

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