Dataset Information

Defining the cellular proteolytic landscape

ABSTRACT: Mass spectrometry analysis of cellular targets of protein degradation

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Assaf Kacen

LAB HEAD: Yifat Merbl

PROVIDER: PXD009360 | Pride | 2018-10-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	QEP2_YME_448_1_071216.raw	Raw
	QEP2_YME_449_1_071216.raw	Raw
	QEP2_YME_450_1_071216.raw	Raw
	QEP2_YME_451_1_071216.raw	Raw
	QEP2_YME_452_1_071216.raw	Raw

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Publications

Revealing the cellular degradome by mass spectrometry analysis of proteasome-cleaved peptides.

Wolf-Levy Hila H Javitt Aaron A Eisenberg-Lerner Avital A Kacen Assaf A Ulman Adi A Sheban Daoud D Dassa Bareket B Fishbain-Yoskovitz Vered V Carmona-Rivera Carmelo C Kramer Matthias P MP Nudel Neta N Regev Ifat I Zahavi Liron L Elinger Dalia D Kaplan Mariana J MJ Morgenstern David D Levin Yishai Y Merbl Yifat Y

Nature biotechnology 20181022

Cellular function is critically regulated through degradation of substrates by the proteasome. To enable direct analysis of naturally cleaved proteasomal peptides under physiological conditions, we developed mass spectrometry analysis of proteolytic peptides (MAPP), a method for proteasomal footprinting that allows for capture, isolation and analysis of proteasome-cleaved peptides. Application of MAPP to cancer cell lines as well as primary immune cells revealed dynamic modulation of the cellula ...[more]

PMID: 30346940

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