Dataset Information

Probing the Sensitivity of the Lumos Mass Spectrometer using a Standard Reference Protein in a Complex Background

ABSTRACT: we determined the sensitivity of the Lumos mass spectrometer using the NIST monoclonal antibody reference material at various concentrations to detect its peptides in a background of S. cerevisiae whole cell lysate, which was kept at a constant concentration.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Laurence Florens

LAB HEAD: Laurence Florens

PROVIDER: PXD009441 | Pride | 2018-09-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Sc_WCL-250ng-100pgNIST_rKCTi_3hRP-20cm_DE30_OL-FTFT_1.ms2	Other
	Sc_WCL-250ng-100pgNIST_rKCTi_3hRP-20cm_DE30_OL-FTFT_1.mzid.gz	Mzid
	Sc_WCL-250ng-100pgNIST_rKCTi_3hRP-20cm_DE30_OL-FTFT_1.pride.mgf.gz	Mgf
	Sc_WCL-250ng-100pgNIST_rKCTi_3hRP-20cm_DE30_OL-FTFT_1.pride.mztab.gz	Mztab
	Sc_WCL-250ng-100pgNIST_rKCTi_3hRP-20cm_DE30_OL-FTFT_1.raw	Raw

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Probing the Sensitivity of the Orbitrap Lumos Mass Spectrometer Using a Standard Reference Protein in a Complex Background.

Levy Michaella J MJ Washburn Michael P MP Florens Laurence L

Journal of proteome research 20180913 10

The use of mass spectrometry as a tool to detect proteins of biological interest has become a cornerstone of proteomics. The popularity of mass spectrometry-based methods has increased along with instrument improvements in detection and speed. The Orbitrap Fusion Lumos mass spectrometer has recently been shown to have better fragmentation and detection than its predecessors. Here, we determined the sensitivity of the Lumos using the NIST monoclonal antibody reference material at various concentr ...[more]

PMID: 30180573

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