Dataset Information

Human colon LC-MSMS

ABSTRACT: Colorectal cancer were diagnosed by colonoscopy and pathology and collected at after surgery.The colorectal cacner proteomes were analyzed for 6 normal mucosal tissues and 6 colorectal cancer mucosal tissues by liquid-chromatography mass spectrometry

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Min Li

LAB HEAD: Geng Tian

PROVIDER: PXD009475 | Pride | 2024-05-21

REPOSITORIES: Pride

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Publications

Glucagon Enhances Chemotherapy Efficacy By Inhibition of Tumor Vessels in Colorectal Cancer.

Xu Yuxue Y Ni Feixue F Sun Daxi D Peng Yue Y Zhao Yaxuan Y Wu Xiaojun X Li Shasha S Qi Xiangyu X He Xinkang X Li Min M Zhou Yizi Y Zhang Chao C Yan Miao M Yao Cuifang C Zhu Shuaishuai S Yang Yang Y An Baijiao B Yang Chunhua C Zhang Guilong G Jiang Wenguo W Mi Jia J Chen Xinju X Wei Pengfei P Tian Geng G Zhang Yin Y

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20231210 6

Chemotherapy is widely used to treat colorectal cancer (CRC). Despite its substantial benefits, the development of drug resistance and adverse effects remain challenging. This study aimed to elucidate a novel role of glucagon in anti-cancer therapy. In a series of in vitro experiments, glucagon inhibited cell migration and tube formation in both endothelial and tumor cells. In vivo studies demonstrated decreased tumor blood vessels and fewer pseudo-vessels in mice treated with glucagon. The comb ...[more]

PMID: 38072640

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Project description:Colorectal cancer often arises from adenomatous polyps. Polyps can grow and progress to cancer, but may also remain static in size, regress, or resolve. Prediciting which progress to cancer and which remain benign is difficult. We developed a long-lived murine model of colorectal cancer with tumors that can be followed by colonoscopy. Our aim was to assess whether these tumors have similar growth patterns and histologic fates to human colorectal polyps to identify features to aid in risk stratification of colonic tumors. Long-lived Apcin/+ mice were treated with 4% dextran sodium sulfate to promote colonic tumorigenesis. Tumor growth patterns were characterized by serial colonoscopy, and pathology was determined. Serial biopsies of tumors were obtained for immunohistochemistry and gene expression profiling by microarray analysis with Affymetrix Whole Genome array. Tumors (n=424) grew, remained static, regressed, or resolved over time with different relative frequencies. Newly developed tumors demonstrated dynamic growth patterns with higher rates of growth and resolution, while more established tumors tended to remain static in size. Colonic tumors were hyperplastic lesions (3%), adenomas (73%), intramucosal carcinomas (20%), or adenocarcinomas (3%). Differentially expressed genes between adenomas and intramucosal carcinomas were identified. We did not identify differentially expressed genes between early and late biopsies from the same tumor. This novel murine model of intestinal tumorigenesis develops colonic tumors that can be monitored by serial colonoscopy, mirror growth patterns seen in human colorectal polyps, and progress to colorectal cancer. Further characterization of cellular and molecular features are needed to determine which features can be used to risk-stratify polyps for progress to colorectal cancer and potentially guide prevention strategies. F1 (SWR x C57BL/6) Apcin/+ mice who had been given two treatments of 4% dextran sodium sulfate in drinking water at weaning underwent colonoscopy around 80 days of age. Those with distal colonic tumors amenable to biopsy had two biopsies taken at that time. Tumors were monitored by colonoscopy every 14 days. Every 28 days, two additional biopsies were taken of each tumor. This protocol was repeated until mice were moribund. Six tumors were removed after sacrifice. The earliest and latest biopsies of each tumor were selected for RNA extraction and Affymetrix hybridization.

			Action	DRS
	MaxQuant_Output.zip	Other
	Z10-1.fasta	Fasta
	Z10-1.msf	Msf
	Z10-1.raw	Raw
	Z10-2.fasta	Fasta