Proteomics

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Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor

ABSTRACT: G protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by mediating a GDP to GTP exchange in the Gα subunit. This leads to dissociation of the heterotrimer into Gα-GTP and Gβγ dimer. The Gα-GTP and Gβγ dimer each regulate a variety of downstream pathways to control various aspects of human physiology. Dysregulated Gβγ-signaling is a central element of various neurological and cancer-related anomalies. However, Gβγ also serves as a negative regulator of Gα that is essential for G protein inactivation, and thus has the potential for numerous side effects when targeted therapeutically. Here we report a llama-derived nanobody (Nb5) that binds tightly to the Gβγ dimer. Nb5 responds to all combinations of β- and γ-subtypes and competes with other Gβγ-regulatory proteins for a common binding site on the Gβγ dimer. Despite its inhibitory effect on Gβγ-mediated signaling, Nb5 has no effect on GTP-bound Gαq and Gαs-mediated signaling events in the environment of a living cell.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Sahil Gulati  

LAB HEAD: Krzysztof Palczewski

PROVIDER: PXD009503 | Pride | 2018-10-19

REPOSITORIES: pride

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In-gelChymotrypsindigest.mgf Mgf
In-gelChymotrypsindigest.msf Msf
In-gelChymotrypsindigest.raw Raw
In-gelTypsindigest.mgf Mgf
In-gelTypsindigest.msf Msf
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Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor.

Gulati Sahil S   Jin Hui H   Masuho Ikuo I   Orban Tivadar T   Cai Yuan Y   Pardon Els E   Martemyanov Kirill A KA   Kiser Philip D PD   Stewart Phoebe L PL   Ford Christopher P CP   Steyaert Jan J   Palczewski Krzysztof K  

Nature communications 20180518 1

G protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by mediating a GDP to GTP exchange in the Gα subunit. This leads to dissociation of the heterotrimer into Gα-GTP and Gβγ dimer. The Gα-GTP and Gβγ dimer each regulate a variety of downstream pathways to control various aspects of human physiology. Dysregulated Gβγ-signaling is a central element of various neurological and cancer-related anomalies. However, Gβγ also serves as a negative regulator of Gα that is essential for G  ...[more]

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