Dataset Information

CXMS BS3 Ras/BRAF/MEK/1433 complex

ABSTRACT: RAF-family kinases are activated by recruitment to the plasma membrane by GTP-bound RAS, where they initiate signaling through the MAP kinase cascade. Here we crosslinked (BS3), digested (trypsin), and analyzed via LC-MS/MS KRAS (human, residues 1-169) bound to intact BRAF (human) in an autoinhibited state with MEK1 (human, ) and a 14-3-3 (SF9) dimer . Analysis of this KRAS/BRAF/MEK1/14-3-3 complex reveals both interprotein and intraprotein crosslinks.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human) Spodoptera Frugiperda

SUBMITTER: Anna Schmoker

LAB HEAD: Michael Eck

PROVIDER: PXD042584 | Pride | 2023-10-24

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	KRAS-BRAF-MEK-1433_sequences.fasta	Fasta
	ams_00252.mgf	Mgf
	ams_00252.raw	Raw
	ams_00252_ProteinProspector_cross-linked_spectra.txt	Txt
	ams_00252_pLink2_cross-linked_spectra.csv	Csv

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Publications

Cryo-EM structure of a RAS/RAF recruitment complex.

Park Eunyoung E Rawson Shaun S Schmoker Anna A Kim Byeong-Won BW Oh Sehee S Song Kangkang K Jeon Hyesung H Eck Michael J MJ

Nature communications 20230729 1

RAF-family kinases are activated by recruitment to the plasma membrane by GTP-bound RAS, whereupon they initiate signaling through the MAP kinase cascade. Prior structural studies of KRAS with RAF have focused on the isolated RAS-binding and cysteine-rich domains of RAF (RBD and CRD, respectively), which interact directly with RAS. Here we describe cryo-EM structures of a KRAS bound to intact BRAF in an autoinhibited state with MEK1 and a 14-3-3 dimer. Analysis of this KRAS/BRAF/MEK1/14-3-3 comp ...[more]

PMID: 37516774

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