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Malus x domestica chloroplast LC-MS/MS

ABSTRACT: Characterization of Malus x domestica chloroplast proteome

INSTRUMENT(S): maXis

ORGANISM(S): Malus Domestica

TISSUE(S): Plant Cell

SUBMITTER: Perttu Haimi

LAB HEAD: Perttu Haimi

PROVIDER: PXD009527 | Pride | 2022-03-01

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	281474976716324.mgf	Mgf
	281474976716324.pride.mgf.gz	Mgf
	281474976716325.mgf	Mgf
	281474976716325.pride.mgf.gz	Mgf
	281474976716326.mgf	Mgf

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1 - 5 of 33

Publications

Differential Chloroplast Proteomics of Temperature Adaptation in Apple (Malus x domestica Borkh.) Microshoots.

Morkūnaitė-Haimi Šarūnė Š Vinskiene Jurgita J Stanienė Gražina G Haimi Perttu P

Proteomics 20191001 19

Temperature stress is one of the most common external factors that plants have to adapt to. Accordingly, plants have developed several adaptation mechanisms to deal with temperature stress. Chloroplasts are one of the organelles that are responsible for the sensing of the temperature signal and triggering a response. Here, chloroplasts are purified from low temperature (4° C), control (22° C) and high temperature (30° C) grown Malus x domestica microshoots. The purity of the chloroplast fraction ...[more]

PMID: 31430045

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Malus x domestica microshoot chloroplast proteome at different growth temperatures

Project description:Chloroplasts were purified by centrifugation in Percoll gradient from low temperature (4 C), control (22 C) and high temperature (30 C) grown Malus x domestica microshoots. The proteins were digested using filter aided sample processing (FASP) and analyzed using nano-LC MS/MS. Constant scan rate of 2 Hz and range from 100 to 2,200 m/z was used for the MS spectra acquisition. Data-dependent auto-MS/MS method (Compass 1.5 acquisition and processing software, Bruker Daltonics) was used for selecting the 10 most abundant precursor ions for fragmentation. The MS/MS summation time was adjusted based on precursor intensity within the range 1-10 Hz. Active exclusion of already analyzed precursors was used with a release time of 30 s. Precursor ion isolation window and the CID collision energy were adjusted between 3-9 Th and 34-55 eV as a function of the m/z value, respectively. The data files (9 low temperature samples, 12 control samples and 9 high temperature sampes) are described in the "metadata.txt" file.

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